Speaker
Description
Nowadays antibiotic resistance is defined by the World Health Organization (WHO) as one of the biggest treat for human health. [1] In the absence of substantial new antibiotic discovery, drug delivery systems (DDS) can be used to transport and release a biologically active compound at the needed site. [2-3] Among several nanocarriers used for drug delivery, mesoporous silica nanoparticles (MSNs) present several advantages. For example, they present an high surface area (up to 1000 m2/g) and they can be easily functionalized with chemical groups which allow to increase, delay, and localize drug release at cell targets. [3] To date, to increase MSNs biocompatibility and increase their stability polymer coated nanoparticles are under study. [4-5]
In this work, MSNs were functionalized separately with two amine groups, triethylenetetramine (TETA) and 3-aminopropyltriethoxysilane (APTES) to give MSN-TETA and MSN-NH2 prior poly-L-lysine (PLL) modification. After functionalization, the flavonoid quercetin was loaded into MSNs. Structure and function were determined by a wide range of techniques such as TEM, SAXS, TGA, FTIR, N2-adsorption/desorption isotherms, DLS and ELS. Drug release was assayed at different conditions (pH and drug loadings) giving release values within the range of drug concentration (2-10 µg/mL) in plasma after an oral administration dose of 200-500 mg of quercetin. Preliminary microbiological assays were also performed indicating a better efficacy of the DDS against Gram positive bacteria.
References
[1] S. Hernando-Amado, T.M. Coque, F. Baquero, Nat Microbiol, 2019, 4, 1432–1442
[2] M. Vallet-Regí, D, Lonzano, B. Gonzalez et al. Advanced Healthcare Materials, 2020, 9, 2000310
[3] RR. Castillo, M. Vallet-Regí, Int J Nanomedicine, 2021;16:4409-4430
[4] G.G. Abdo, M.M. Zagho, A.Khalil, Emergent Materials, 2020, 3, 407–425.
[5] K. Kuldeep,K.Deepak.,A. Rosling, J. Rosenholm,Applied Science, 2020, 10, 1, 289
