BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Key talk: Designed Protein Cages: Current State and Potential Medi cal Applications DTSTART;VALUE=DATE-TIME:20220715T090000Z DTEND;VALUE=DATE-TIME:20220715T092500Z DTSTAMP;VALUE=DATE-TIME:20260720T195832Z UID:indico-contribution-57-758@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Jonathan Heddle\; Malopolska Centre of Biotechnology \, Poland ()\nDesigned proteins offer us the potential of matching or exce eding the capabilities of nature’s own naturally-occurring protein nanom achines which demonstrate a wonderous array of properties. Protein cages a re hollow\, typically spherical nanoscale protein assemblies which in natu re have multiple uses such as materials storage\, genome delivery (viruses ) and catalysis (enzymes). As such it is attractive to design and produce artificial cages with properties such as multiple antigen display on the e xterior for use as vaccines\, and the ability to protect and carry therape utically useful macromolecules in the interior for use as drug delivery sy stems. Making such systems programmable is a desirable feature and require s finding a way of opening such cages on demand to release cargo when and where required. In this presentation I will give an overview of the field and our own progress in producing a programmable artificial protein cage.1 \,2\,3\,4\,5\,6\n\nReferences\n1. Malay\, A. D. et al. An ultra-stable gol d-coordinated protein cage displaying reversible assembly. Nature 569\, 43 8–442 (2019).\n2. Malay\, A. D. et al. Gold Nanoparticle-Induced Formati on of Artificial Protein Capsids. Nano Lett. 12\, 2056–2059 (2012).\n3. Imamura\, M. et al. Probing structural dynamics of an artificial protein c age using high-speed atomic force microscopy. Nano Lett. 15\, 1331–1335 (2015).\n4. Naskalska\, A. et al. An artificial protein cage delivers acti ve protein cargoes to cell interior. Biomacromolecules 22\, 4146–4154 (2 021).\n5. Stupka\, I. et al. Chemically induced protein cage assembly with programmable opening and cargo release. 9424\, (2022).\n6. Majsterkiewicz \, K. et al. Artificial Protein Cage with Unusual Geometry and Regularly E mbedded Gold Nanoparticles. Nano Lett. 22\, 3187–3195 (2022).\n\nhttps:/ /indico.koza.if.uj.edu.pl/event/7/contributions/758/ LOCATION:Collegium Novodvorscianum URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/758/ END:VEVENT BEGIN:VEVENT SUMMARY:Invited talk: Perspectives of preclinical research in Bialystok Ce nter of Molecular Imaging DTSTART;VALUE=DATE-TIME:20220715T092500Z DTEND;VALUE=DATE-TIME:20220715T094500Z DTSTAMP;VALUE=DATE-TIME:20260720T195832Z UID:indico-contribution-57-716@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Anna Gromotowicz-Poplawska\; Medical University of B ialystok\, Poland ()\nBialystok Center of Molecular Imaging (BCMI) is one of the key elements of the new project of the Innovative Research Center f or the Prevention of Civilization Diseases and Individualized Medicine (CB I PLUS)\, that is now proceeding by the Medical University of Bialystok (M UB). This will be the first laboratory for the synthesis and quality contr ol of radiopharmaceuticals in the region and the first PET/MR preclinical research center in Poland. The main goal of BCMI is to develop new radioph armaceuticals for the early diagnosis and treatment of civilization diseas es. This will be supported by the preclinical and clinical research with t he innovative molecular imaging technique application. The BCMI labs will follow the GMP and GLP procedures. At the moment\, the construction of the BCMI is underway. The GMP documentation has already been prepared and the purchase of the hot cell\, synthesis unit\, 68Ge/68Ga generator and quali ty control system is ongoing. In the preclinical research labs the PET/MR and SPECT/CT scanners for small animals will be installed. The configurati on of systems will allow for a whole body scan\, brain\, heart\, lung and amdominal imaging in preclinical research in the field of oncology\, neuro logy\, cardiology and metabolic diseases. The animal scanners will be a pa rt of the unique imaging system that is already used in the MUB (i.a. huma n PET/MR hybrid system)\, thus providing the translational research. BCMI will increase opportunities for collaboration and conducting multicentre r esearch as well as international projects. The entire BCMI installation sh ould be completed in mid-2023.\n\nhttps://indico.koza.if.uj.edu.pl/event/7 /contributions/716/ LOCATION:Collegium Novodvorscianum URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/716/ END:VEVENT BEGIN:VEVENT SUMMARY:Proteomic profiling of extracellular vesicles derived from pancrea tic beta-cells cultured under hyperglycemia DTSTART;VALUE=DATE-TIME:20220715T094500Z DTEND;VALUE=DATE-TIME:20220715T100500Z DTSTAMP;VALUE=DATE-TIME:20260720T195832Z UID:indico-contribution-57-717@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Carina Rząca\; Jagiellonian University\, Poland ()\ nCargo carried by extracellular vesicles (EVs) is considered as a promisin g diagnostic marker\, especially proteins [1\,2]. EVs can be divided accor ding to their size and way of biogenesis into exosomes (diameter <200 nm) and ectosomes (diameter <200 nm) [1].\nFirst step of this study was charac terization of EVs sample [2]. Using Tunable Resistive Pulse Sensing (qNano ) size distribution and concentration were measured. The mean size of exos omes was 135±15 nm\, and ectosomes 210±17 nm. TEM images also confirmed the size of EVs and the purity of prepared samples. In the present study\, a nano liquid chromatography with tandem mass spectrometry (nanoLC-MS/MS) was used to profile and compare the protein content of ectosomes and exos omes secreted by pancreatic beta cells (1.1B4) grown under NG (5 mM D-gluc ose) and HG (25 mM D-glucose) conditions. The EVs samples were lysed\, and proteins were denaturated\, digested\, and analyzed using a Q-Exactive ma ss spectrometer coupled with the UltiMate 3000 RSLC nano system (Thermo Fi sher Scientific) The LC-MS/MS data were searched against SwissProt Homo sa piens database using MaxQuant software and protein quantitation was done b y the MaxLFQ algorithm. Statistical analysis was carried out with Perseus software. Further bioinformatic analysis was performed using the FunRich 3 .1.4 software with the UniProt protein database and STRING [3\, 4\, 5].\nA s a result of the tandem mass spectrometry analysis more than 1\,000 prote ins were identified and quantified in each sample. The average number of i dentified proteins in exosomes and ectosomes was 1\,397 and 1\,697\, respe ctively. Label-free quantitative analysis showed that exosome and ectosome protein composition differed significantly between those isolated under N G and HG conditions. Many pathways were down-regulated in HG\, particularl y the ubiquitin-proteasome pathway. In addition\, a significant up-regulat ion of the Ras-proteins pathway was observed in HG.\nOur description of EV s protein content and its related functions provides the first insight int o the EV interactome and its role in hyperglycemia development and diabeti c complications. The results also indicate the applicability of some of th ese EVs proteins for further investigation regarding their potential as ci rculating in vitro biomarkers [6\, 7\, 8].\n\nReferences\n[1] Stępień E Ł\, et. al. Bio-Algorithms and Med-Systems 2021\;17\,4\; 301-309.\n[2] Ka mińska A\, PhD Thesis 2019\, Kraków\, WFAIS UJ.\n[3] Surman M\, et. al. International Journal of Molecular Science 2021\, 22(13)\, 6816.\n[4] Surm an M\, et. al. Cells 2022\, 11(7)\, 1184.\n[5] Surman M\, et. al. Proteome s 2019\, 7(2)\,21.\n[6] Stępień E\, et. al. Theranostics 2018\;8(14).\n[ 7] Stępień E\, et al. 2021\, Patent Nr: PAT.237810.\n[8] Stępien E\, et al. 2020\, Patent Nr: PAT.235682.\n\nhttps://indico.koza.if.uj.edu.pl/eve nt/7/contributions/717/ LOCATION:Collegium Novodvorscianum URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/717/ END:VEVENT BEGIN:VEVENT SUMMARY:Key talk: PET/CT and SPECT/CT in preclinical research: Systems and applications DTSTART;VALUE=DATE-TIME:20220715T083500Z DTEND;VALUE=DATE-TIME:20220715T090000Z DTSTAMP;VALUE=DATE-TIME:20260720T195832Z UID:indico-contribution-57-715@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Sibylle Ziegler\; Ludwig-Maximilian University of Mu nich\, Germany ()\nPET and SPECT are truly translational since the same me thods can be applied in animals and in clinical studies. Owing to the smal l structures\, dedicated instrumentation is needed for mouse and rat imagi ng\, while large animal models can be investigated using clinical systems. Some new detector concepts have been introduced first in preclinical syst ems before scaling up to clinical systems. Biodistribution and biokinetics of novel radioactively labelled tracers are studied in rodents before tra nslating into patient use. Multi-tracer longitudinal measurements can be p erformed for characterizing disease models or assessing the effect of inte rventions. In addition\, similar quantitative image analysis methods can b e applied in preclinical models before translating to the clinical case.\n In this presentation\, an overview on specific instrumentation for animal imaging will be given\, together with examples of preclinical imaging stud ies performed in our lab with specific focus on specific tracer applicatio ns in neurology and beta-cell transplant research.\n\nhttps://indico.koza. if.uj.edu.pl/event/7/contributions/715/ LOCATION:Collegium Novodvorscianum URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/715/ END:VEVENT END:VCALENDAR