BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Invited talk: Nuclear Imaging in Infective Endocarditis DTSTART;VALUE=DATE-TIME:20220711T094500Z DTEND;VALUE=DATE-TIME:20220711T100500Z DTSTAMP;VALUE=DATE-TIME:20260720T190632Z UID:indico-contribution-40-656@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Kostkiewicz\; Nuclear Medicine Department\ , John Paul II Hospital\, Poland ()\nInfective endocarditis is a complex d isease with cardiac involvement and multiorgan complications. Its prognosi s depends on prompt diagnosis that leads to an aggressive therapeutic mana gement combining antibiotic therapy and early cardiac surgery when indicat ed. However\, IE diagnosis always poses a challenge. In recent years\, nuc lear imaging ( technetium99m hexamethylpropyleneamine oxime-labelled autol ogous leukocytes (99mTc-HMPAO-SPECT/CT) and 18FDG PET) have experienced si gnificant technical improvements\, and their application to the detection of cardiac and extracardiac IE-related lesions seems to be a strategic way forward in the management of patients with suspected IE. The accumulation of time-dependent radiolabelled leucocytes (99mTc-HMPAO-WBC) is registere d to evaluate in vivo inflammatory lesions. The main added value of this t echnique is its high specificity and detection of peripheral embolic event s. Sensitivity and specificity of 99mTc-HMPAO-SPECT/CT was 86% and 97%\, r espectively. Positron emission tomography with fluorodeoxyglucose (18F-FDG PET) technology delivers high-resolution images by the use of biologicall y active compounds labeled with positron emitters. Uptake of the radiophar maceutical in PET is based on the high expression of glucose transporters\ , which actively incorporate the tracer into the cells. A radiolabeled gly cogen analog accumulates into cells such as activated inflammatory cells i n infection and inflammation processes.Metabolic activity is evaluated bot h qualitatively and quantitatively. If PET/CT acquisition is combined with cardiac CT (PET/CT)\, the metabolic findings provided by the [18F]FDG upt ake distribution and intensity might be added to the anatomic findings alr eady described for cardiac CT within a single imaging procedure. Notable a dvantages of PET/CT and WBC SPECT/CT are their ability to perform the extr acardiac workup within a single imaging procedure and to reveal the concom itant presence of extracardiac infection sites as the consequence of both septic embolism and primary infective processes. Nuclear examination may p rovide opportunities for personalized evaluation in order to choose the be st therapeutic strategy.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contr ibutions/656/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/656/ END:VEVENT BEGIN:VEVENT SUMMARY:Invited talk: Molecular imaging of human stem/progenitor cells for pro-regenerative purposese DTSTART;VALUE=DATE-TIME:20220711T090500Z DTEND;VALUE=DATE-TIME:20220711T092500Z DTSTAMP;VALUE=DATE-TIME:20260720T190632Z UID:indico-contribution-40-580@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Maciej Kurpisz\; Institute of Human Genetics\, Polis h Academy of Sciences\, Poland ()\nWe have used human stem/progenitor cell s of myogenic origin (with mostly CD56+\, desmin-positive characteristics) either alone or together with mesenchymal stem cells (MSC) to be applied in the mouse post-infarction heart model (immunocmpromise SCID mice) when tracking them in situ in medium and long-term imaging system. In order to differentiate between myoblasts anbd MSC/s we have used two types of biolu minescent markers (firefly liciferase versus nanoluc) with two different m olecular promoters incorporated to the stem cells while delivering the cel ls intramyocardially to post-infarcted heart using four variants of cellul ar therapies. We have found by bioluminescent imaging that in a group of m ice with post-infarcted heart the highest signal was obtained when myoblas ts were applied together with MSC/s vs myoblasts alone (p<0.0001). This co uld be a promising strategy for pro-regenerative future clinical trials.Fo r a long cell imaging we additionally used [18F}-FHBG PET/CT model when ap plying stem cells with molecular double promoter/reporter sequence that co uld either link flushed 18F isotopes with FDG (fluorodeoxyglucose) for cel l viability. In vivo PET/CT and MRI revealed precise measurement of report er probes signaling incorporated into the cells for as long as 6 weeks of monitoring.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/580/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/580/ END:VEVENT BEGIN:VEVENT SUMMARY:Invited talk: Quantitative analysis of tumor hypoxia in nuclear me dicine imaging and therapy DTSTART;VALUE=DATE-TIME:20220711T092500Z DTEND;VALUE=DATE-TIME:20220711T094500Z DTSTAMP;VALUE=DATE-TIME:20260720T190632Z UID:indico-contribution-40-655@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kuangyu Shi\; University of Bern\, Switzerland ()\nT umour hypoxia is a critical hallmark of cancer\, which is associated with tumour aggressiveness and resistance to multiple therapies. PET imaging me thods have been developed for noninvasive visualization of tumour hypoxia and the emerging radioligand therapy is expected to overcome the limitatio n of conventional external beam radiotherapy in cancer treatment. However\ , it is not straightforward to interpret the PET imaging signals for tumou r hypoxia due to the complex tumour microenvironment. On the other side\, the influence of hypoxia on radioligand therapy is more complex than the p enetration distance of therapeutic radioisotopes. This talk will give an o verview of computational methods including pharmacokinetic modelling and r eaction-diffusion modelling in the interpretation of hypoxia in PET imagin g and radioligand therapy. The combination of experimental data and comput ational modelling may accelerate the development of hypoxia-related nuclea r medicine imaging and therapy.\n\nhttps://indico.koza.if.uj.edu.pl/event/ 7/contributions/655/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/655/ END:VEVENT END:VCALENDAR