BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:Development of the normalization method for the Jagiellonian PET s canner DTSTART;VALUE=DATE-TIME:20220712T090500Z DTEND;VALUE=DATE-TIME:20220712T090700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-781@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Aurélien Coussat\; Jagiellonian University\, Poland ()\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/781/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/781/ END:VEVENT BEGIN:VEVENT SUMMARY:Estimation of 511 keV gamma scatter fraction in WLS layer in Total Body J-PET \; A simulation study DTSTART;VALUE=DATE-TIME:20220712T083700Z DTEND;VALUE=DATE-TIME:20220712T083900Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-744@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Keyvan Tayefi Ardebili\; Jagiellonian University\, P oland ()\nAuthor: Keyvan Tayefi Ardebili\nCo-authors: Szymon Niedżwiecki \, Paweł Moskal\nEstimation of 511 keV gamma scatter fraction in WLS laye r in Total Body J-PET\n\nKeyvan Tayefi Ardebili1\,2\, Szymon Niedżwiecki 1\,2\, Paweł Moskal 1\,2 on behalf of the J-PET collaboration.\n\n1Facult y of Physics\, Astronomy\, and Applied Computer Science\, Jagiellonian Uni versity\, Łojasiewicza 11\, 30-348 Kraków\, Poland\n2Center for Theranos tics\, Jagiellonian University\, Cracow\, Poland\n\nAbstract:\nA positron emission tomography (PET) scan plays an essential role in medical diagnost ics and monitoring therapy. A new generation of Total-Body PET scanners ba sed on plastic scintillators is being developed by the J-PET collaboration at Jagiellonian University [1].\nThe total body J-PET scanner comprises o f 7 rings\, each ring consisting of 24 modules. A single module is built o f 2 layers each one 16 axially arranged plastic scintillator strips of 33 cm in length\, read out by silicon photomultiplier (SiPM) arrays from both ends\, and an additional layer of 50 wavelength shifter (WLS) fibers. In this study\, an estimation of the scatter fraction of the Total-Body J-PET manufactured from plastic scintillator strips according to the NEMA NU 2- 2018 standards by using GATE software. The scatter phantom was simulated a s a solid cylinder with a length of 700 mm and an outside diameter equal t o 203 mm while at a radial distance of 45 mm we have a hole with a diamete r of 6.4 mm that linear source with total activity 1 MBq is placed [2\,3]. For data processing\, sinograms were generated and the Single Slice Rebin ning (SSRB) algorithm was used for the calculated scatter fraction amount. \nAcknowledgments:\nThe authors acknowledge support by the TEAM POIR.04.04 .00-00-4204/17 program\, the NCN grant no. 2021/42/A/ST2/00423 and the Sci Mat and qLife Priority Research Areas budget under the program Excellence Initative - Research University at the Jagiellonian University.\nReference s:\n1. P. Moskal\, et al. "Simulating NEMA characteristics of the modular total-body J-PET scanner - an economic total-body PET from plastic scintil lators"\, Phys. Med. Biol. 66 (2021) 175015.\n2. P. Kowalski\, et al. “E stimating the NEMA characteristics of the J-PET tomograph using the GATE p ackage”\, Phys. Med. Biol. 63 (2018) 165008\n3. P. Kowalski\, et al. “ Scatter fraction of the J-PET tomography”\, Acta Phys. Pol. B 47 (2016) 549\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/744/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/744/ END:VEVENT BEGIN:VEVENT SUMMARY:Characterization of the 192-strip J-PET detector for multi-photon positronium imaging DTSTART;VALUE=DATE-TIME:20220712T085700Z DTEND;VALUE=DATE-TIME:20220712T085900Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-775@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kamil Dulski\; Jagiellonian University\, Poland ()\n Author: K. Dulski on behalf of the J-PET collaboration\n\nPositronium imag ing is a promising new technique that can enhance the diagnostic capabilit ies of Positron Emission Tomography (PET)\, based on a new structural inde x derived from ortho-positronium interaction with the environment in which it annihilates [1\,2]. A positronium (Ps) can be formed during a standard PET scan when a positron emitted from a radiopharmaceutical administered to a patient forms a bound state with the electron. Depending on the total spin number S\, the positronium can be formed in one of two states - para -Ps (p-Ps\, S = 0) and ortho-Ps (o-Ps\, S = 1). These two states differ ma inly in the average lifetime (in vacuum: 0.125 ns for p-Ps and 142 ns for o-Ps) and the number of photons that are emitted during the annihilation ( p-Ps even\, o-Ps odd number of photons). In particular\, the lifetime of o -Ps may be shortened when interacting with the environment in which it is formed. Therefore\, by simultaneously reconstruction of the position of o- Ps annihilation and its average lifetime\, it becomes possible to characte rize the structure of a given part of the sample in space\, which is the b asis for positronium imaging [1]. Currently\, the J-PET detector [1-3] is the only detector that is able to obtain positronium images. The positroni um images of the two phantoms measured by the 192-strip J-PET detector wil l be shown [1\,4]. Additionally\, data on the sensitivity and purity of tw o- and three-photon positronium imaging will be presented on the basis of simulation data [4].\n[1] P. Moskal\, K. Dulski\, N. Chug et al.\, Positro nium imaging with the novel multiphoton PET scanner\, Science Advances 7 ( 2021) eabh4394\n[2] P. Moskal and E.Ł. Stępień\, Positronium as a bioma rker of hypoxia\, Bio-Aglorithms and Med-Systems 17 (2021) 311-319\n[3] P. Moskal\, S. Niedźwiecki\, T. Bednarski et al.\, Test of a single module of the J-PET scanner based on plastic scintillators. Nucl. Instr. and Meth . A 764\, 317-321 (2014)\n[4] K. Dulski\, PhD Thesis: Development of posit ronium imaging with the 192-strip J-PET detector (2022)\n\nhttps://indico. koza.if.uj.edu.pl/event/7/contributions/775/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/775/ END:VEVENT BEGIN:VEVENT SUMMARY:Towards improving the sensitivity of testing CPT symmetry in posit ronium decays with the Modular J-PET detector DTSTART;VALUE=DATE-TIME:20220712T090300Z DTEND;VALUE=DATE-TIME:20220712T090500Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-777@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Neha Chug\; Jagiellonian University\, Poland ()\nAut hor: Neha Chug\n\nNeha Chug1\,2* on behalf of the J-PET Collaboration\n\n1 Faculty of Physics\, Astronomy and Applied Computer Science\, Jagiellonian University\, Krakow\, Poland\n2Center for Theranostics\, Jagiellonian Uni versity\, Krakow\, Poland\n*e-mail: neha.chug@doctoral.uj.edu.pl\n\nThe Ja giellonian Positron Emission Tomograph is the first plastic scintillator b ased tomographic device used to test discrete symmetries in the charged le ptonic sector [1]. One of such tests is for CPT symmetry\, under the combi ned transformation of charge\, parity and time reversal\, in the decays of positronium atoms [2]. J-PET performed its first measurement for CPT symm etry test by searching for non vanishing CPT-violating angular correlation s between the spin and orientation of decay plane of ortho-positronium (o- Ps) atoms\, which is the triplet state of positronium [3\, 4]. Sensitivity of testing CPT symmetry with the J-PET detector reaches the precision lev el of 10-4 [4]. Here we will discuss the prospects of improving the sensit ivity of this test beyond the level of 10-4 by enhancing the photon regist ration efficiency using a new layer of densely packed plastic scintillator s and a spherical annihilation chamber as a positronium production medium [5].\n\nAcknowledgement:\nThe authors acknowledge support by the TEAM POIR .04.04.00-00-4204/17 program\, the NCN grant no. 2019/35/B/ST2/03562 and 2 021/42/A/ST2/00423\, and the SciMat and qLife Priority Research Areas budg et under the program Excellence Initiative - Research University at the Ja giellonian University.\n\nReferences:\n[1] P. Moskal et. al.\, Acta Phys. Polon. B 47\, 509 (2016)\n[2] B. K. Arbic et al.\, Phys. Rev. A 37\, 3189 (1988)\n[3] A. Gajos et al.\, Acta. Phys. Pol. A 137\, 126 (2020).\n[4] P. Moskal\, A. Gajos et. al.\, Nature Communications\, 12\, 5658 (2021)\n[5] A. Gajos\, Symmetry\, 12(8)\, 1268 (2020)\n\nhttps://indico.koza.if.uj.ed u.pl/event/7/contributions/777/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/777/ END:VEVENT BEGIN:VEVENT SUMMARY:Assessment of the influence of the Beta parameter in the reconstru ction of Q.Clear DTSTART;VALUE=DATE-TIME:20220712T085100Z DTEND;VALUE=DATE-TIME:20220712T085300Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-771@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Konrad Skórkiewicz\; Jagiellonian University\, Pola nd ()\nAuthor: Konrad Skórkiewicz\nCo-authors: Anna Sowa-Staszczak\, Kazi mierz Łątka\n\nAim/Introduction: The aim of the study is to determine th e appropriate value of the β parameter using the Q.Clear reconstruction a lgorithm in the imaging of patients with neuroendocrine tumors. Materials and Methods: The analysis concerned the measurements of the NEMA IEC Body Phantom\, filled with Ga-68 gallium chloride. Within the phantom we placed the 4 smalles hot spheres filled with a higher isotope concentration in c omparison with the body part. Imaging was performed in the PET/CT scanner in few time intervals. The raw data were reconstructed with the use of the Q.Clear reconstruction algorithm with 18 values of the β parameter (150- 1000\, every 50). Results: The obtained results show that together with an increase of the values of the β parameter\, the image quality in the Q.C lear reconstruction algorithm increases. Referring to the scientific repor ts\, one can see that the signal to noise ratio in the image increases. Th e effect of the change of the β parameter on the SUV mean value is the la rgest for the smallest sphere. The percentage decrease is much higher also with the lower values of the activity\, reaching a value of 3.7% and 8.5% for large and small sphere with β=450\, in comparison with β=200. With β=1000 a very significant decrease is observed\, especially for the small est sphere and for the lowest activity measured\, which is 18.5 % when β= 200. Conclusion: An increase of the values of the β parameter has an adve rse effect on the quantitative assessment of SUV. In the visual assessment \, a satisfactory image quality is present with β=450. This value results in a relatively low decrease of the SUV mean and SUV max.\n\nhttps://indi co.koza.if.uj.edu.pl/event/7/contributions/771/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/771/ END:VEVENT BEGIN:VEVENT SUMMARY:Detection of concentration and survival of HL-60 human acute promy elocytic leukemia cells by the PALS technique DTSTART;VALUE=DATE-TIME:20220712T084300Z DTEND;VALUE=DATE-TIME:20220712T084500Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-747@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Katsiaryna Yankova\; Maria Curie-Skłodowska Univers ity\, Poland ()\nAuthor: Katsiaryna Yankova\nCo-authors: Bożena Zgardziń ska\, Bożena Jasińska\, Marek Gorgol\, Marcin Czop\, Janusz Kocki\n\nThe HL-60 human acute promyelocytic leukemia cells obtained from the Clinical Genetics Department of Medical University in Lublin were investigated wit h the use of positron and positronium probes. The HL-60 cell line is a pop ular and convenient test object due to easy reproduction. The cell line co mes from the patient\, 36-year-old Caucasian female with acute promyelocyt ic leukemia. The 16 samples were used for the study: 15 with HL-60 cell-li ne with different concentration of cells and 1 sample with a pure medium\, as a reference. The studies were performed with positron annihilation lif etime spectroscopy (PALS) and the results were correlated with cell surviv al. Examined samples were subjected to various external factors during mea surements: ionizing radiation\, time and light.\nIn contrast to our expect ations\, we found that the o-Ps component in PALS spectra does not differe ntiate the samples sufficiently and can’t be used as the only analyzed p arameter. The components of para-positronium and free annihilation should also be considered. Our studies indicate the possibility of assessing the concentration of cell lines using the PALS technique\, but a much more imp ortant factor differentiating the obtained results turned out to be the co ncentration of live cells in the sample. The positron annihilation shows a very close correlation with viability of the cells in the samples. Relayi ng on gathered information we can conclude that the PALS technique can be used as a highly sensitive method for estimating the quantity of living ce lls in sample.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/7 47/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/747/ END:VEVENT BEGIN:VEVENT SUMMARY:Intelligent data analysis for the next generation medical phantom Dose-3D DTSTART;VALUE=DATE-TIME:20220712T082500Z DTEND;VALUE=DATE-TIME:20220712T082700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-738@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kamila Kalecińska\; AGH University of Science and T echnology\, Poland ()\nAuthors: Kamila Kalecińska\, Dose-3D Collaboration \n\nMedical data intelligent analysis is the part of TEAM NET \nDose3D Pro ject “A Reconfigurable Detector for Measuring the Spatial Distribution o f Radiation Dose for Applications in the Preparation of Individual Patient Treatment Plans”. The goal of the Dose3D consortium is to build a three -dimensional measurement system containing a detector capable of directly measuring the spatial distribution of the deposited therapeutic dose in re al-time. The keyword “reconfigurable” means that such a tissue-like ph antom can be configured to mimic a specific part of the patient 's body. D elivery of a proper geometry which is in the form of 3D Computed Tomograph y (CT) scans of the patient body with highly precise delineation of the ar ea of affection to the detector is the important step of the whole process . The idea is to build a reliable\, fully automatic tool that would be abl e to extract the desired volume from 3D CT images. Technically this proces s (named segmentation) refers to assigning each voxel of the 3D image to a specific class.\nTraining data for segmentation tasks consists of raw CT scans in DICOM format as well as already manually segmented data in DICOM- RT Structures format. Medical data format complexity and data limitations mostly caused by privacy issues that require the preparation of a special preprocessing pipeline. One of the important steps in this procedure is to perform an effective data augmentation\, which refers to artificially inc reasing the amount of training data. Both data augmentation and 3D image a utomatic segmentation tools will be built on the basis of the most advance d deep learning models.\nHigh computational power and GPU’s support are required in the process of training 3D deep learning models. Fortunately\, the modern platform delivered by NVIDIA (NVIDIA Clara) with Python framew ork MONAI is dedicated to healthcare data analysis. This framework provide s state-of-the-art pre-trained ML models\, a set of tools for medical prep rocessing and domain-specific GPU optimization.\nThe presentation will be about prospects\, challenges and the current stage of developing medical d ata analysis tools with the potential to improve the individual treatment plans.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/738/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/738/ END:VEVENT BEGIN:VEVENT SUMMARY:Double photon coincidence detection method for gamma-ray imaging i n medicine DTSTART;VALUE=DATE-TIME:20220712T082300Z DTEND;VALUE=DATE-TIME:20220712T082500Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-737@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Mizuki Uenomachi\; Kyoto University\, Japan ()\nAuth ors: Mizuki Uenomachi\, Kenji Shimazoe\, Hiroyuki Takahashi\n\nPositron em ission tomography (PET) utilizes the coincidence detection of annihilation gamma-rays with energy of 511 keV produced after a positron-electron coll ision. The positron position can only be constrained on a line connecting the detection points because two annihilation gamma-rays emit at the oppos ite direction. On the other hands\, some nuclides emit successive gamma-ra ys via an intermediate state with a short duration such as 111In\, 177Lu\, 60Co\, and so on. These successive gamma-rays are emitted at almost isotr opic direction\; thus\, the radionuclide location can be identified by usi ng direction-resolving radiation detection system. We have exemplified the position identification capability of double photon coincidence method by applying to Compton imaging and mechanical collimation-based gamma-ray im aging. Moreover\, we have demonstrated its crosstalk reduction capability in multi-nuclide Compton imaging. In the presentation\, we will show the e xperimental results of the double photon coincidence method application.\n \nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/737/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/737/ END:VEVENT BEGIN:VEVENT SUMMARY:Design and Application for a new intense positron beam at the Anti matter Laboratory in Trento DTSTART;VALUE=DATE-TIME:20220712T081500Z DTEND;VALUE=DATE-TIME:20220712T081700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-733@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Luca Povolo\; University of Trento\, Italy ()\nAutho r: Luca Povolo\nCo-authors: Sebastiano Mariazzi\, Luca Penasa\, Ruggero Ca ravita\, Sushil Sharma\, Roberto Sennen Brusa\n\nAt the Anti-Matter Labora tory (AML) of the Department of Physics of the University of Trento a new positron beam is currently under development. It is being constructed for the production into vacuum of Positronium (Ps\, the bound state of an elec tron and the positron\, e+)\, which will be used for fundamental studies\, like inertial sensing [1]. However\, Ps is emitted into vacuum in the gro und state which has a lifetime of only 142 ns\, while studies with positro nium require lifetime in the microsecond range. This becomes possible by e xciting Ps into a metastable state via a two-photon transition\, as we dem onstrated [2].\nThe production of positronium atoms start by injecting pos itron into a target\, called e+/Ps converter [3-6]. The new positron beam of our laboratory is based on a 22Na radioactive source coupled with a sol id noble gas moderator held at cryogenic temperature [7]. Up to now we obt ained a continuous beam with up to 50000 positrons per second per milli-Cu rie. The next step will be the bunching of the continuous beam with a Buff er-Gas Penning trap [8] which will be followed by the positron bunch injec tion into the converter.\nIn this work\, we will present the preliminary d esign of the apparatus with simulations. The extraction of the positron co ntinuous beam from the source-moderator via magnetic field will be discuss ed in detail. The plan for the trapping and injection of the positron will be presented. Moreover\, we will discuss the tests for a possible detecto r for Positronium inertial sensing\, which needs to have the capability of 3D reconstruction of the Ps annihilation point.\n[1] S. Mariazzi et al.\, Eur. Phys. J. D 74\, 79 (2020)\n[2] C. Amsler et al.\, Phys. Rev. A 99\, 033405 (2019)\n[3] S. Mariazzi et al.\, Phys. Rev. B 81\, 235418 (2010)\n[ 4] S. Mariazzi et al.\, Phys. Rev. Lett. 104\, 243401 (2010)\n[5] S. Maria zzi et al.\, J. Phys. B: At. Mol. Opt. Phys. 54\, 085004 (2021)\n[6] S. Ma riazzi et al.\, Phys. Rev B 105\, 115422 (2022)\n[7] A. P. Mills Jr. et al .\, Appl. Phys. Lett. 49\, 1121 (1986)\n[8] R. G. Greaves et al.\, NIM B 1 92 (2002)\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/733/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/733/ END:VEVENT BEGIN:VEVENT SUMMARY:CP Discrete Symmetry study in the decay of ortho-Positronium atom using the J-PET detector. DTSTART;VALUE=DATE-TIME:20220712T090100Z DTEND;VALUE=DATE-TIME:20220712T090300Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-778@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kavya Valsan Eliyan\; Jagiellonian University\, Pola nd ()\nKavya Valsan Eliyan\, Juhi Raj\, On Behalf of the J-PET Collaborati on Jagiellonian University.\n\nFaculty of Physics\, Astronomy and Applied Computer Science\, Jagiellonian University\, Kraków\, Poland.\nTheranosti cs Center\, Jagiellonian University\, Kraków\, Poland.\nEmail: kavya.eliy an@doctoral.uj.edu.pl\, juhi.raj@doctoral.uj.edu.pl\n\nInteraction between electron-positron pair leads to direct annihilation into photons or creat ion of a bound state called Positronium. Positronium is the lightest purel y leptonic object decaying into photons [1-3]. Positronium atom can be for med in two states based on the spin alignment of its constituting particle s\, Singlet state (1S0 -para-Positronium (p-Ps) and Triplet state (3S1- or tho-Positronium (o-Ps). Constrained by conservation laws\, the o-Ps annihi late into odd number of photons (o-Ps → 3γ\, where γ = 1\, 2\, …)\, while the p-Ps decay into an even number of photons (p-Ps → 2γ\, where γ – 1\,2\, 3\, ...) [4\,5]. As an atom bound by a central potential\, i t is a parity eigenstate\, and as an atom built out of an electron and an anti-electron\, it is an eigenstate of the charge conjugation operator [1] . Therefore\, the positronium is a unique laboratory to study CP discrete symmetry involving correlations of photons momenta originating form o-Ps a nnihilation [6]. The Standard Model predicts that the photon–photon inte raction and weak interactions will mimic the symmetry violation in the ord er of 10−9 and 10−13 respectively [6]. Violation of CP invariance in p urely leptonic systems has never been seen so far [7]. The experimental li mits on CP and CPT symmetry violation in the decays of o-Ps are set at the level of 10−3 [2\,8].\n\nIn the year 2021\, the limitations of the prev ious experiments were overcome by the J-PET detector due to its much highe r granularity and improve the world result by a factor of three and reache s the statistical precision of 10−4. The reported result is the present best upper limit on the CP violation in the decay of ortho-Positronium\, l eaving us 5 orders of more statistical sensitivity to be explored in this aspect. J-PET detector is constructed of 192 polymer scintillators\, where each scintillator is attached with photomultipliers at each end. 192 scin tillators are arranged co-axially in three layers at 3 different radii 42. 5 cm\, 46.75 cm\, 57.5 cm respectively. Positronium atom can be formed in the center of J-PET detector using the beta-emitter 22Na source placed ins ide a small chamber. The source is sandwiched between an aerogel material. Plastic scintillators offer high time and angular resolution. Time Over T hreshold is adopted as a measure of energy deposition. The signals are mea sured by using the trigger-less data acquisition [9-12]. All of the previo us investigations with Positronium\, which tested the discrete symmetries\ , were based on symmetry odd operators constructed as the products of phot ons momenta (k⃗ i̇ ) and Positronium spin (S⃗ ̇ ) vectors [2-4\,6]. This project describes an extended study using another proposed operator [ 4]\, taking advantages of properties of the 3 layered J-PET detector\, whi ch enables to determine the linear polarization direction of annihilation photons. Measurement of polarization direction of annihilation photons (51 1 keV) is a unique feature of the J-PET detector which allows the study of CP symmetry violation by determining the expectation values of the CP sym metry odd operator (ε⃗ i⋅.k⃗ j) where i ≠j). As a future prospect \, the J-PET collaboration has developed a modular version of the J-PET de tector to improve the detection efficiency of this measurement and provide larger statistics in a shorter duration of measurement time to improve th e precision significantly.\n\nACKNOWLEDGEMENTS:\nThe author acknowledge su pport by the TEAM POIR.04.04.00-00-4204/17 program\, the NCN grant no. 202 1/42/A/ST2/00423 and the SciMat and qLife Priority Research Areas budget u nder the program Excellence Initiative - Research University at the Jagiel lonian University.\n\nReferences:\n[1] M. Skalsey\, J. Van House\, Phys. R ev. Lett. 67\, 1993 (1991).\n[2] P. A. Vetter\, S.J. Freedman\, Phys. Rev. Lett. 91\, 263401 (2003).\n[3] T. Yamazaki\, T. Namba\, S. Asai\, T. Koba yashi\, Phys. Rev. Lett. 104\, 083401 (2010).\n[4] P. Moskal et al.\, Acta Phys. Polon. B 47\, 509 (2016).\n[5] E.Czerwinski et al.\, Acta Phys. Pol on. B 48\, 509 (2017).\n[6] P. Moskal\, A. Gajos\, M. Mohammed\, Nat. Comm un. 12\, 5658 (2021).\n[7] V. A. Kostelecky and N. Russell\, 2018 update t o Rev. Mod. Phys. 83\, 11 (2011).\n[8] T. Yamazaki\, T. Namba\, S. Asai\, T. Kobayashi\, Phys. Rev. Lett. 104\, 083401 (2010).\n[9] M. Palka\, Jour. Of Instr. 12\, P08001 (2017)\n[10] G. Korcyl et al.\, Acta Phys. Polon. B 47\, 491 (2016).\n[11] Sz. Niedzwiecki et al.\, Acta Phys. Polon. B 48\, 1567 (2017).\n[12] A. Gajos et al.\, Nucl. Instr. and Meth. A 819\, 54 (20 16).\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/778/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/778/ END:VEVENT BEGIN:VEVENT SUMMARY:Measurement of correlation between polarization of annihilation ph otons emitted in e+e- system to detect entanglement at sub-MeV range DTSTART;VALUE=DATE-TIME:20220712T085300Z DTEND;VALUE=DATE-TIME:20220712T085500Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-772@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Deepak Kumar\; Jagiellonian University\, Poland ()\n Author: Deepak Kumar\nCo-author: Sushil Sharma\n\nD. Kumar1\,2\,3\, S. Sha rma1\,2\,3 on behalf of the J-PET collaboration\n1Faculty of Physics\, Ast ronomy\, and Applied Computer Science\, Jagiellonian University\, Poland.\ n2Total-Body Jagiellonian-PET Laboratory\, Jagiellonian University\, Krak ów\, Poland\n3Center for Theranostics\, Jagiellonian University\, Cracow\ , Poland\ne-mail: d.kumar@doctoral.uj.edu.pl\n\nQuantum electrodynamics pr edicts that photons originating from the decays of e+e- annihilations are entangled and have mutually orthogonal linear polarization [1]. Since the polarization of the photons is orthogonal to each other\, correlation can occur in subsequent interactions. Compton scattering of photons can be use d as a polarization analyser to measure such correlations [2]. To measure the correlation between the scattered photon due to entanglement\, the two photons must be detected before and after the scattering [3]. Thanks to i ts unique geometry\, J- PET can be used as a potential detector to perform such studies in full phase space [4\,5\,6]. It consists of 192 plastic sc intillators with dimensions 50 x1.9 x 0.7cm3 (length x width x height) arr anged in 3 cylindrical layers with increasing radial distance 42.50 cm\, 4 6.75 cm and 57.5 cm\, respectively [4]. Photons interact mainly via the Co mpton effect inside plastic scintillators. Since an incident photon intera cting with the plastic scintillator is mostly scattered perpendicular to t he polarization direction of the incident photon\, the polarization of a s ingle photon is defined as the cross product of the momentum vectors of th e photon before and after scattering (ϵ=k ×k') [7].\nOur goal is to stud y the correlation between the polarization vectors of annihilation photons produced either by direct annihilation of electron and positron or by the formation of a positronium atom in the presence of a medium. In this pres entation\, the methodology to perform such studies in the framework of J-P ET detector and the preliminary results will be presented.\nThe authors ac knowledge support by the TEAM POIR.04.04.00-00-4204/17\nprogram\, the NCN grant no. 2021/42/A/ST2/00423 and the SciMat and\nqLife Priority Research Areas budget under the program Excellence\nInitiative - Research Universit y at the Jagiellonian University.\nReferences:\n[1] Snyder\, H. S.\, Paste rnack\, S. & Hornbostel\, J Phys. Rev.73\, 440 (1948)\n[2] O. Klein\, Y. N ishina\, Y. Z. Physik 52\, 853 (1929)\n[3] P. Moskal et al.\, Acta Phys. P olon. B 47\, 509 (2016)\n[4] S. Niedzwiecki et al.\, Acta Phys. Polon. B48 (2017) 1567\n[5] P. Moskal et al.\, Nature Communications 12. 5658 (2021) \n[6] P. Moskal et al.\, Science Advances\, Vol 7\, Issue 42\, Page No. 43 94 (2021)\n[7] P. Moskal et al. Eur. Phys. J. C 78\, 970 (2018).\n\nhttps: //indico.koza.if.uj.edu.pl/event/7/contributions/772/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/772/ END:VEVENT BEGIN:VEVENT SUMMARY:Characterization of spheroid growth based on a new dynamical model DTSTART;VALUE=DATE-TIME:20220712T085500Z DTEND;VALUE=DATE-TIME:20220712T085700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-774@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kamil Dulski\; Jagiellonian University\, Poland ()\n Authors: K. Dulski1\,2 and M. Szczepanek1\,2\n\n1Institute of Physics\, Ja giellonian University\, Kraków\, Poland\n2Center for Theranostics\, Jagie llonian University\, Kraków\, Poland\n\nCell cultures are a recognized mo del that helps understand interaction of cells with certain external facto rs\, such as radiation or drugs [1\,2]. In particular\, characterizing the growth of a given cell line for a different intensity of a therapeutic ag ent allows for non-invasive assessment of its effectiveness [1]\, and in s ome cases also for optimization of therapeutic conditions for cells of a g iven type [3\,4]. Currently\, there are two types of cell culture - 2D\, w here cells grow in a planar monolayer and 3D\, an example of which are sph eroids. 3D cultures are characterized by greater similarities to tumours i n the conditions occurring in the body. The common features between spher oids and tumours allow for a more complete understanding evaluation of the effectiveness of therapy [3-6]. \nCurrently\, the growth of biological sy stems is mainly described by logistic models\, most often with the Gompert z model [7\,8]. However\, this model does not always describe the experime ntal data perfectly\, and it also fails to characterize the cell line base d on parameters such as cell size\, nutrient consumption\, and separation zones of strongly dividing cells from zones with dead and non-dividing cel ls. A new\, dynamic model of spheroid growth will be presented\, allowing to characterize the above-mentioned parameters and additionally better ref lecting the spheroid growth curve. Additionally\, the simulations performe d using dedicated software allowed for a detailed characterization of the WM266-4 skin cancer cell line\, as well as for the theoretical visualizati on of the distribution of various zones inside the spheroid at different g rowth times.\n[1] D.A. Scudiero\, R.H. Shoemaker\, K.D. Paull et al.\, Eva luation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug S ensitivity in Culture Using Human and Other Tumor Cell Lines\, Cancer Res. 48 (1988) 4827-4833\n[2] M.F. Bernet\, B. Brassart\, J.R. Nesser and A.L. Servin\, Lactobacillus acidophilus LA 1 binds to cultured human intestina l cell lines and inhibits cell attachment and cell invasion by enterovirul ent bacteria\, Gut 35 (1994) 483-489\n[3] M. Zoetemelk\, M. Rausch\, D.J. Colin et al.\, Scientific Reports 9 (2019) 7103\n[4] M. Silarski\, K. Dzie dzic-Kocurek and M. Szczepanek\, Combined BNCT and PET for theranostics\, Bio-Algorithms and Med-Systems 17 (2021) 293-300\n[5] E.Ł. Stępień\, H. Karimi\, B. Leszczyński and M. Szczepanek\, Melanoma spheroids as a mode l for cancer imaging study\, Acta Phys. Pol. B 51 (2020) 159-163\n[6] M. S zczepanek\, Application of 3D model of cancer cellsin research on the effe ctiveness of BNCT in the treatment of melanoma\, Acta Phys. Pol. B 51 (202 0) 413-419\n[7] R.L. Buchanan\, R.C. Whiting\, W.C. Damert\, When is simpl e good enough: a comparison of the Gompertz\, Baranyi\, and three-phase li near models for fitting bacterial growth curves\, Food Microbiology 14 (19 97) 313-326\n[8] R. Omar\, M.A. Abdullah\, M.A. hasan\, M. Rosfarizan and M. Marziah\, Kinetics and modelling of cell growth and substrate uptake in Centella asiatica cell culture\, Biotech. Bioprocess Eng. 11 (2006) 223-22 9\n\nAcknowledgment\nThis work was supported by the Foundation for Polish Science through the MPD and TEAM/2017-4/39 programs\, the National Science Centre of Poland through grant nos. 2017/25/N/NZ1/00861\, 2019/35/B/ST2 / 03562 and 2021/41/N/ST2/03950\, the Jagiellonian University via project CR P/ 0641.221.2020\, and the SciMat Priority Research Area budget under the program Excellence Initiative-Research University at Jagiellonian Universi ty\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/774/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/774/ END:VEVENT BEGIN:VEVENT SUMMARY:Development of a high-resolution PET detector for small animal in- beam PET system DTSTART;VALUE=DATE-TIME:20220712T085900Z DTEND;VALUE=DATE-TIME:20220712T090100Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-776@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Munetaka Nitta\; Ludwig Maximilians University\, Ger many ()\nAuthors: Munetaka Nitta\, Giulio Lovatti\, Kang Han Gyu\, Rohgieh Haghani\, Chiara Gianoli\, Georgious Dedes\, Andrea Zogular\, Yamaya Taig a\, Christoph Scheidenberger\, Marco Durante\, Peter Thirolf\, Katia Paodi \n\nWe have been developing a high resolution small in-beam PET system in the framework of the project “Small animal Irradiation for Research in M olecular Image-guided radiation Oncology (SIRMIO)”\, which is an EU-fund ed endeavor aiming to realize a prototype platform for accurate image-guid ed small animal proton irradiation at clinical facilities. In this project we plan to deliver a proton beam to the mouse tumor and monitor the posit ron emitters generated by the beam using a dedicated in-beam PET scanner. The PET scanner exhibits a unique spherical shape for high detection sensi tivity along with sufficient open space for accommodating the beam and int egrating additional beam monitoring detectors as well as a mouse holder. I n addition to that\, uniform sub-millimeter spatial resolution is required for accurate range verification. In order to achieve these requirements\, in a collaborative effort between LMU and QST\, we have developed a 3-lay ers depth-of-interaction (DOI) PET detector [1]. The PET detector is compo sed of LYSO scintillator pixels with 0.9 mm×0.9 mm×6.67 mm size read out by an 8×8 SiPM array. A charge division circuit is used to reduce the 64 signals of the SiPM array to 4 signals for an Anger calculation. 56 PET d etectors are embedded in a spherical housing frame.\nIn this study\, a poi nt source experiment was carried out to evaluate the spatial resolution of the PET scanner especially in the central region of the field of view and along beam axis. There\, we could achieve the targeted 1 mm spatial resol ution\, confirming satisfactory performance for our project. In addition t o that\, we will show the capability of our PET detector for high resoluti on radioactive ion beam imaging of C-11\, as explored in the context of th e EU-funded project “Biomedical Applications of Radioactive Beams” (BA RB).\n\nAcknowledgment\;\nThis work is funded by the European Research Cou ncil (ERC) under the European Union's Horizon 2020 research and innovation programme through the grant agreements number 725539 (SIRMIO\, PI K. Paro di) and 883425 (BARB\, PI M. Durante). The authors would also like to ackn owledge the support from the Bavaria California Technology Center (grant A 1 [2021-1]). Part of the results are based on an experiment carried out in the context of FAIR Phase-0 at GSI\, Darmstadt (Germany).\n\nReference\n[ 1] 2021\, Kang et al.\, BPEX\, vol 7\, no. 3\, pp. 035018\n\nhttps://indic o.koza.if.uj.edu.pl/event/7/contributions/776/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/776/ END:VEVENT BEGIN:VEVENT SUMMARY:Relevance of Monte Carlo simulation validation analysis in the sco pe of the Dose-3D project DTSTART;VALUE=DATE-TIME:20220712T084900Z DTEND;VALUE=DATE-TIME:20220712T085100Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-770@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Wioleta Górska\; AGH University of Science and Tech nology\, Poland ()\nAuthors: Wioleta Górska\, DOSE-3D Collaboration\n\nRa diotherapy aims to deliver a specific dose of radiation to the treated are a\, destroying the tumour. Thanks to the use of newer technologies and the ir continuous development\, it is possible to very precisely deliver the p lanned doses of radiation to the treated areas\, while reducing the exposu re of healthy tissues to radiation. Undoubtedly\, physical simulations and the use of algorithms supporting the work of medical physicists contribut ed to the improvement of the quality of treatment. Due to their advantages \, MC simulations are used both in the area of treatment planning and scie ntific research. However\, to obtain reliable calculation results\, it is necessary to previously validate the MC simulations\, concerning experimen tal and reference data. This milestone has been already achieved in the sc ope of the Dose-3D project and this presentation is aimed to present its s ummary.\nThe Dose-3D project is a novel reconfigurable detector intended f or a full spatial therapeutic dose reconstruction to improve radiotherapy treatment planning. The main challenge of the project is the construction of a 3D measurement matrix. One of the crucial challenges is the developme nt of high-quality software for dose simulation\, configuration and contro l of the entire device\, and data analysis. A key issue in the research wi ll be to obtain a high agreement between the simulated dose distributions and those measured during the detector tests. For this purpose\, The GEANT 4-RT simulator platform was assembled. The implementation of a reliably fu nctioning platform using the GEANT4 engine to simulate the interaction of radiation with matter is crucial for the success of the entire project.\nT o verify the reliability of the modelled Varian Clinac 2300 C/D medical li near accelerator head and used physics model in the GEANT4-RT the spatial dose distributions were calculated and then correlated with the respective measurements provided by The Maria Sklodowska-Curie National Research Ins titute of Oncology in Krakow. The comparisons were conducted using a varie ty of methods including the common and standard verification approaches us ed in clinical practice like dose difference analysis and gamma test. The validation was extended by comparison with the results generated by Penelo pe engine based MC simulator - PRIMO\, whose reliability was verified in a ddition to Geant4-RT scope of studies. Once the geometry specification of linac’s is not publicly available we rely on the linac model defined in the PRIMO simulator. Extracting phaspace for the given beam model from PRI MO we continued simulation in GEANT4-RT application including field modell ing here.\nFurthermore\, MC simulation allows for physics model specificat ions and tuning values correlated with it like production cut. For this re ason\, it is necessary to verify the reliability of selected configuration s. To execute such validation\, an analysis of the photon interactions in the water phantom was performed.\nIn conclusion\, MC simulation platform w ithin the Dose-3D project has been initialised and verified as the in-hous e application for further development for reconfigurable scintillator phan tom purposes.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/77 0/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/770/ END:VEVENT BEGIN:VEVENT SUMMARY:Application of the PALS technique in the investigation of the nano structure of enzymatic biosensor matrices for biomarkers detection in medi cal diagnostics DTSTART;VALUE=DATE-TIME:20220712T082100Z DTEND;VALUE=DATE-TIME:20220712T082300Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-736@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Goździuk\; Maria Curie-Skłodowska Univer sity\, Poland ()\nAuthor: Magdalena Goździuk\nCo-authors: Bożena Zgardzi ńska\, Taras Kavetskyy\n\nBiosensors are devices widely used in many fiel ds of medicine. Using biosensors we can detect biomarkers of pathological states. One of very popular biosensor types are enzymatic biosensors. The biggest advantage of enzymatic biosensor is detection of specific molecule s depending on the enzyme used which is associated with the absence of fal se positives. Sensors of this type are characterized by high sensitivity a nd selectivity. Enzymatic biosensors have a matrix which improves biosenso r detecting abilities and immobilize an enzyme. Matrices can be produced f rom many types of materials e.g. gel\, carbon nanotubes\, chitosan\, nanow ires\, polymers etc.\nWe use Positron Annihilation Lifetime Spectroscopy t o investigate nanostructure of biosensors biopolymer matrices based on soy bean oil. The matrices are the part of amperometric enzymatic biosensors. The advantage of using polymer matrices is their low resistance so they do not interfere with the detection mechanism. The second important issue is good liquid sorption to a matrix to get better sensitivity. Soybean- oil based matrices were investigated in wide range of temperatures (120 K÷320 K) to determine the phase transitions in samples and analyze nanostructur e changes due to temperature. The next stage of research was sorption/ des orption using deionized water\, NaCl solution and polluted water. Using PA LS we can forecast matrix properties used in biosensor construction basing on ortho-Positronium lifetimes (τ3) and intensities (I3) changes under g iven humidity conditions and correlate results received using other invest igation methods.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions /736/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/736/ END:VEVENT BEGIN:VEVENT SUMMARY:Calibration of Silicon Drift Detectors for the SIDDHARTA-2 Experim ent DTSTART;VALUE=DATE-TIME:20220712T081700Z DTEND;VALUE=DATE-TIME:20220712T081900Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-734@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Aleksander Khreptak\; National Laboratory of Frascat i (LNF)\, Italy ()\nAuthor: Aleksander Khreptak\n\nThe main aim of the SID DHARTA-2 experiment at the DAΦNE collider in the LNF-INFN (Italy) is to p erform the high precision measurement of the kaonic deuterium exotic atom\ , which is formed when a negatively charged kaon (K−) is captured in a h ighly atomic excited state\, replacing an electron [1\,2].\n\nTo achieve t his goal\, a large area Silicon Drift Detectors (SDDs) system has been dev eloped by the SIDDHARTA-2 Collaboration [3]. The energy response of each d etector should be calibrated and monitored to reduce the systematic error (to the level of 2-3 eV).\n\nThe poster will present a calibration method for the SIDDHARTA-2 setup [4]\, which should guarantee high precision spec troscopic performances of the system during the data taking.\n\n[1] M. Mil iucci et al.\, Il Nuovo Cimento 44 C (2021) 152.\n[2] C. Curceanu et al.\, Few-Body Syst. 62 (2021) 83.\n[3] M. Miliucci et al.\, Meas. Sci. Technol . 32 (2021) 095501.\n[4] F. Sgaramella et al.\, arXiv:2201.12101 (2022).\n \nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/734/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/734/ END:VEVENT BEGIN:VEVENT SUMMARY:Gold nanoparticles as contrast agents for micro-CT imaging DTSTART;VALUE=DATE-TIME:20220712T084700Z DTEND;VALUE=DATE-TIME:20220712T084900Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-755@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Dominik Panek\; Jagiellonian University\, Poland ()\ nAuthors: Dominik Panek\, Monika Szczepanek\nCo-authors: Bartosz Leszczyń ski\, Paweł Moskal\, Ewa Stępień\n\nMicro-computed tomography (micro-CT ) is nowadays often used to examine biological samples. This technique\, b ased on the attenuation of X-rays\, is capable of achieving micrometric re solution. However\, the challenge is to stain the samples in such a way th at they will be opaque to the X-ray radiation with a given energy. That is why different types of contrast agents are being currently developed in o rder to obtain the highest contrast. But the contrast itself is often not enough for the biological studies. It is also important for the contrastin g agent not to be toxic in any way – it has to be biocompatible. Example of such contrasting agents are gold nanoparticles (AuNPs)\, which are by far the most studied nanomaterials and\, amongst other metallic nanopartic les\, are believed to be the most appropriate for biological research [1\, 2]. In our studies\, the CT scanner (SkyScan 1127) was used in order t o visualize the uptake and accumulation of AuNPs. The nanoparticles were i ncubated with melanoma cell line (WM266-4) spheroids – a 3D cell model o f cell culture intended to imitate tumor tissue\, especially the environme nt inside it [3]. In this case\, micro-CT was used to check if it is possi ble to visualize AuNPs inside spheroids created from a the same number of cells (2000) and on different days of growth (3rd and 7th)\, incubated for 24h. The concentration of gold NPs used in this study was identical for a ll the samples and equal to 2.5 µg/ml. Additioanlly\, the same concentrat ion of AuNPs was added to the cells from the beginig of spheroid creation to comapre it with standard method. The results of experiments indicate th at AuNPs are promising contrast agents\, due to their high atomic numer\, which does not require the use of high concentraions. The spheroid was not created in case of the method where AuNPs were added to the cells from th e beginning.\nReferences\n[1] Kus-Liśkiewicz M\, et al. Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies a nd Regulations. Int J Mol Sci. 2021\;22(20):10952.\n[2] Karimi\, H.\, Lesz czyński\, et al.\, X-ray microtomography as a new approach for imaging an d analysis of tumor spheroids. Micron\, 137.\n[3] Stępień\, E.\, Karimi\ , H.\, Leszczyński\, B.\, & Szczepanek\, M. (2020). Melanoma spheroids as a model for cancer imaging study. Acta Physica Polonica. B\, 51(1)\, 159 –163.\nAcknowledgements\nThe authors acknowledge support by the TEAM POI R.04.04.00-00-4204/17 program and the SciMat and qLife Priority Research A reas budget under the program Excellence Initiative - Research University at the Jagiellonian University and by DSC grant\, no. N17/MNS/000058/2021\ , awarded to D. Panek funded by Jagiellonian University.\n\nhttps://indico .koza.if.uj.edu.pl/event/7/contributions/755/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/755/ END:VEVENT BEGIN:VEVENT SUMMARY:Study of differences in the composition of glycosphingolipids betw een the extracellular vesicles from β-cell and endothelium cell lines usi ng ToF-SIMS DTSTART;VALUE=DATE-TIME:20220712T084500Z DTEND;VALUE=DATE-TIME:20220712T084700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-748@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Marzec\; Jagiellonian University\, Poland ()\nAuthor: Dr Magdalena Marzec\nCo-authors: Carina Rząca\, Ewa Stepien\n \nTime of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is used to ana lyze biomolecules in tissues\, cells and membranous structures. This type of mass spectrometry enables qualitative semi-native testing without the n eed for isolation\, fixation or labelling of target elements with simultan eous 2D imaging. The analyzed mass range is less than 1000 Da\, which make s it possible to study of amino acids and lipids [1]. Extracellular vesicl es (EVs) are spherical cellular structures surrounded by a lipid bilayer i n size from 30 to 1000 nm. The basic classification of EVs distinguishes t hree subpopulations: exosomes derived from endosomes (50–150 nm)\, cell ectosomes (100–1000 nm) and apoptotic bodies (1000–5000 nm). Due to th e constantly growing importance of EVs in the diagnosis and treatment of d iseases\, more and more attempts are made to effectively isolate\, detect and analyze them [2]. So far\, it has not been demonstrated whether the in creased sugar concentration in the external environment modifies the compo sition of glycosphingolipids (GSL) in the composition of cell membranes an d EVs.\nThe aim of the study is to compare the GSL composition in EV subpo pulations: ectosomes and exosomes. In this study\, EVs were purified by a low-pressure filtration and concentrated by an ultracentrifugation. EVs co me from two cell lines: pancreatic β-cells and microvascular endothelium cells (TIME). Both cell lines were grown under normoglycemic (NG) and hype rglycemic (HG) conditions. The experiment also investigated the change in the content of the analyzed biomolecules in EVs due to high glucose concen tration influence.\nAs a result of the conducted research\, we have shown that we can perform glycosphingolipid analysis in extracellular vesicles u sing ToF-SIMS. The analysis showed significant changes in GSL composition depending on the cell line (β-cells and microvascular endothelium cells) for both ectosome and exosome populations. Cell culture conditions (hyperg lycemia) affect the glycosphingolipids profile both in the group of ectoso mes and exosomes.\n\nReferences:\n[1] M.E. Marzec\, D. Wojtysiak\, K. Poł towicz\, J. Nowak\, R. Pedrys\, Study of cholesterol and vitamin E levels in broiler meat from different feeding regimens by TOF-SIMS\, Biointerphas es. 11 (2016). https://doi.org/10.1116/1.4943619.\n[2] A. Kamińska\, M. R oman\, A. Wróbel\, A. Gala-Błądzińska\, M.T. Małecki\, C. Paluszkiewi cz\, E.Ł. Stępień\, Raman spectroscopy of urinary extracellular vesicle s to stratify patients with chronic kidney disease in type 2 diabetes\, Na nomedicine Nanotechnology\, Biol. Med. 39 (2022) 102468. https://doi.org/h ttps://doi.org/10.1016/j.nano.2021.102468.\n\nAcknowledgements\nThis work has been funded from the SciMat and qLife Priority Research Area budget un der the Strategic Programme Excellence Initiative at the Jagiellonian Univ ersity and by the National Science Center (NCN)\, grant OPUS 17 to prof. E . Stępień (No. 2019/33/B/NZ3/01004).\n\nhttps://indico.koza.if.uj.edu.pl /event/7/contributions/748/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/748/ END:VEVENT BEGIN:VEVENT SUMMARY:Determination of 10B concentration in melanocytes and melanoma cel ls DTSTART;VALUE=DATE-TIME:20220712T084100Z DTEND;VALUE=DATE-TIME:20220712T084300Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-746@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Monika Szczepanek\; Jagiellonian University\, Poland ()\nAuthor: Monika Szczepanek\nCo-authors: Anna Telk\, Ewa Stępień \ n\nMelanoma is the most aggressive skin cancer\, difficult to treat when m etastatic. It is also cancer that may become a candidate for Boron Neutron Capture Therapy (BNCT). BNCT is type of radiation therapy that employ the altered metabolism of cancer cells and additionally minimizes side effect s. During treatment\, the patient is administered a compound containing th e non-radioactive isotope of boron (10B)\, which accumulates in the melano ma cells due to its increased metabolism compared to normal cells [1]. The n\, the diseased area of the patient's body is irradiated with a thermal n eutron beam. As a result of irradiation\, cells with accumulated 10B are d estroyed\, while neighboring cells\, not loaded with 10B stayed undamaged. Selectivity in this therapy is based on the increased metabolism of neopl astic cells\, which ensures a higher boron concentration compared to norma l cells [2\,3]. The principle of efficient BNCT depends on the amount of b oron delivered to cancer cells \, which must be 3 times higher in compare to normal cells. Therefore\, the aim of proposed research is to assess the uptake of the 10B isotope by normal and cancer cells in an 2D in vitro mo del.\nIn our research\, we used normal skin cells – melanocytes and two melanoma cell lines\, delivered from primary tumor (WM115) and metastasis (WM266-4). Cells were incubated with boron carrier (boronophenylalanine\, BPA) in concentration 50 µg B/ mL\, for 2\, 4\, 6 and 12 hours. The effec tive boron carrier ensures a high concentration of 10B atoms in the cells at the level of 20-35 µg10B / g of tumor tissue ( 1.2-2.1x109 10B atoms p er cell) [4]. After incubation cells were harvested and 10B isotope concen tration was measured using inductively coupled plasma mass spectrometry (I CP-MS).\nBoth melanoma cell lines reached maximum concentration of 10B aft er 4h of incubation with BPA\, 9\,2 µg10B/g and 27\, 9 µg10B/g for WM115 and WM266-4\, respectively and these concentrations seemed to fluctuate w ithin 12 hours of incubation. In case of melanocytes 10B concentration in cells increased during the incubation time up to reach the highest boron c oncentration at 33\,7 µg10B/g in 12 hour of incubation.\nOur results show ed different kinetics of boron uptake in different cell types which is in confirms previous studies [5]. These data suggest the need to investigate the mechanism of BPA uptake and metabolism in other melanoma cell lines to eligible BPA for BNCT cancer treatment.\nReferences:\n[1] Mishima Y\, Hon da C\, Ichihashi M\, Obara H\, Hiratsuka J\, Fukuda H\, et al. Treatment o f malignant melanoma by single thermal neutron capture therapy with melano ma-seeking 10B-compound. Lancet. 1989\;2:388–389.\n[2] Nedunchezhian K\, Aswath N\, Thiruppathy M\, Thirugnanamurthy S. Boron Neutron Capture Ther apy - A Literature Review. J Clin Diagn Res.2016\;10:ZE01-04.\n[3] Silarsk i M\, Dziedzic-Kocurek K\, Szczepanek M. Combined BNCT and PET for therano stics. BAMS. 2021\;17:293-300.\n[4] Verlinden B\, Van Hoeckeet K\, Aertsal A\, Daems N\, Dobney A\, Janssens K\, et al. Quantification of boron in c ells for evaluation of drug agents used in boron neutron capture therapy. JAAS. 2021\;36:598-606 .\n[5] Carpano M\, Perona M\, Rodriguez C\, Nievase t S\, Olivera M\, Santa Cruz G.A\, al. Experimental Studies of Boronopheny lalanine (10BPA) Biodistribution for the Individual Application of Boron N eutron Capture Therapy (BNCT) for Malignant Melanoma Treatment Int. J. Rad iat. Oncol. Biol. Phys. 2015\;93:344-352.\nAcknowledgments:\nThis work was supported by the SciMat Priority Research Area budget under the Strategic Programme Excellence Initiative at the Jagiellonian University through gr ant No. U1U/P05/NO/03.47.\n\nhttps://indico.koza.if.uj.edu.pl/event/7/cont ributions/746/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/746/ END:VEVENT BEGIN:VEVENT SUMMARY:Breast Cancer diagnosis study along with the introduction of new d etection technology DTSTART;VALUE=DATE-TIME:20220712T083900Z DTEND;VALUE=DATE-TIME:20220712T084100Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-745@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Shivani\; Jagiellonian University\, Poland (Jagiello nian University)\nAuthor: Shivani\n\nShivani1\,2 On the behalf of J-PET co llaboration\n1Faculty of Physics and Applied Computer Science\, Jagielloni an University\, Cracow\, Poland\n2Center for Theranostics\, Jagiellonian U niversity\, Poland\n\nIn both developing and developed countries\, breast cancer is the top cause of mortality among women. Medical imaging plays an important role for breast cancer screening\, for classifying and examinin g indistinct breast abnormalities\, as well as for defining the extent of breast tumors [1]. Positron Emission Mammography is one of the most widely used imaging modalities today (PEM). The goal of the J-PET group is to de velop\, build\, and test the J-PEM (Jagiellonian Positron Emission Tomogra phy)\, which is based on a novel concept using plastic scintillators[2\,3\ ,4\,5] and a wavelength shifter (WLS) [6\,7]. readout.\n\nThe results of t he examination of data acquired from the hospital\, which included 131 les ions\, will be presented in this poster. The cases involved 114 individual s\, with 98 having one lesion\, 14 having two lesions\, and one patient ha ving three lesions. The findings of the BI RADS-based diagnostic test will be presented. A comparison of ROC curves will also be shown.\nThe perform ance of a newly designed J-PEM scanner prototype is also characterised in this work. The goal of the J-PET group is to develop\, build\, and test th e J-PEM (Jagiellonian Positron Emission Tomography)\, which is based on a novel idea using plastic scintillators[2\,3\,4\,5] and wavelength shifter (WLS) [6\,7] readout. The prototype system is made up of two layers of pla stic scintillators (6x24x500 mm) and one layer of wavelength shifters [3] (3x10x100 mm) arranged orthogonally between them. For signal reading\, eac h scintillator bar is connected to Silicon Photomultipliers on both ends. This three-dimensional device is based on the innovative notion of using p lastic scintillators to detect annihilation photons and wavelength shifter s to improve spatial resolution (WLS). Gate simulation was used to calcula te the point spread function\, sensitivity\, and scatter fraction. To unde rstand the difference\, simulations were run with WLS strips (Z = 1.28 mm) and without WLS strips (Z = 10 mm). It is apparent that utilising WLS str ips lowered the value of PSF along the Z-axis by about half (4.7 mm).\n\n[ 1] E. Łuczyńska\,et al.\, Med Sci Monit\, 2015\; 21: 1358-1367\n[2] P. M oskal\, Sz. Niedźwiecki\, et al.\, Nucl. Instr. Meth. A 764\, 317 (2014). \n[3] P. Moskal\, O. Rundel\, et al.\, Phys. Med. Biol. 61\, 2025 (2016).\ n[4] P. Moskal\, K. Dulski\, et al.\, Science Advances 7 (2021) eabh4394.\ n[5] P. Moskal\, A. Gajos\, et al.\, Nature Communications 12 (2021) 5658. \n[6] J. Smyrski\, P. Moskal\, et al.\, BioAlgorithms and Med-Systems 10\, 59 (2014).\n[7] J. Smyrski\, et al.\, Nuclear Inst. and Methods in Physic s Research A 851\, 39-42\,\n(2017).\n\nAcknowledgements:\nThe authors ackn owledge support by the TEAM POIR.04.04.00-00-4204/17 program\, the NCN gra nt no. 2021/42/A/ST2/00423 and the SciMat and qLife Priority Research Area s budget under the program Excellence Initiative - Research University at the Jagiellonian University\n\nhttps://indico.koza.if.uj.edu.pl/event/7/co ntributions/745/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/745/ END:VEVENT BEGIN:VEVENT SUMMARY:Developing a phantom for the positronium imaging evaluation. DTSTART;VALUE=DATE-TIME:20220712T083500Z DTEND;VALUE=DATE-TIME:20220712T083700Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-743@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Gabriela Łapkiewicz\; Jagiellonian University\, Pol and ()\nAuthor: Gabriela Łapkiewicz\nCo-authors: Pawel Moskal\, Szymon Ni edźwiecki\n\nG. Łapkiewicz1\,2*\, Sz. Niedźwiecki1\,2\, P. Moskal1\,2\, on behalf of J-PET collaboration\n\n1Faculty of Physics\, Astronomy and A pplied Computer Science\, Jagiellonian University\, Cracow\, Poland\n2Cent er for Theranostics\, Jagiellonian University\, Cracow\, Poland\n\nPhantom s used in PET technique\, such as NEMA IEC allow for measurement of activi ty concentration accumulated in different volumes. New imaging method deve loped by the Jagiellonian PET collaboration in addition to the annihilatio n density distribution enables measurement of positronium lifetime [1-4]. To qualitatively determine the precision of this method it is essential to construct a phantom\, which will allow for measuring ortho-positronium li fetime alongside activity concentration.\n\nThe proposed phantom (much lik e NEMA IEC) will consist of 6 volumes of high activity accumulation immers ed in the lower activity background. Each volume will feature different me an\nlifetime of ortho-positronium. Isotopes used for measurements must not only exhibit β+ activity\, but also need to emit prompt gamma quanta (i. e. 44Sc or68G a) [5]\, [6]. In this contribution a\nmethod for controlling ortho-positronium lifetime is discussed along with preliminary results.\n \nIn order to evaluate a method for the prepration of media with different ortho-positronium lifetime we have studied the ortho-positronium lifetime in water suspension of XAD4 porous material.\nXAD4 is characterized with the average pore size of 50 Å and can absorb water up to 60% of its mass [7].\n\nFive samples of XAD4 with controlled amount of water were measured using PALS technique. Additionally one dry sample of XAD4 and one sample of pure water were measured. Obtained spectra were fitted with PALS Avalan che [8] and components corresponding to the ortho- positronium annihilatio n in XAD4 pores were established [9]. The results showed the correlation b etween the lifetime and production intensity of ortho-positronium and the concentration of XAD4 in water.\n\nAcknowledgements:\nThe authors acknowle dge support by the TEAM POIR.04.04.00-00-4204/17 program\, the NCN grant n o. 2021/42/A/ST2/00423 and the SciMat and qLife Priority Research Areas bu dget under the program Excellence Initiative - Research University at the Jagiellonian University.\n\nReferences:\n[1] P. Moskal et al.\, Positroniu m imaging with the novel multiphoton PET scanner\, Science Advances 7 (202 1)\n[2] P. Moskal\, Positronium Imaging\, 2019 IEEE Nuclear Science Sympos ium and Medical Imaging Conference (NSS/MIC) (2019) pp. 1-3\n[3] P. Moskal et al.\, Feasibility study of the positronium imaging with the J-PET tomo graph\, Phys. Med. Biol. 64 055017 (2019)\n[4] P. Moskal\, E. Ł. Stępie ń.\, Positronium as a biomarker of hypoxia\, Bio-Algorithms and Med- Syst ems 17 (2021) 311-319\n[5] T. Matulewicz\, Radioactive nuclei for β+γ PE T and theranostics: selected candidates\, Bio- Algorithms and Med-Systems 17 (2021) 235-239\n[6] J. Choiński\, M. Łyczko\, Prospects for the produ ction of radioisotopes and radiobioconjugates for theranostics\, Bio-Algor ithms and Med-Systems 17 (2021) 241-257\n[7] Sigma-Alrdrich\, XAD4 specifi cations sheet: www.sigmaaldrich.com/specification-sheets/ 304/271/XAD4-BUL K___SIGMA____.pdf\n[8] K. Dulski\, PALS Avalanche - A New PAL Spectra Anal ysis Software\, Acta. Phys. Pol. A 137 (2020) 167\n[9] K.Dulski\, The J-PE T detector-a tool for precision studies of ortho-positronium decays\, Nucl ear Inst. and Methods in Physics Research\, A 1008 (2021) 165452\n*Corresp onding author\, email: gabriela.lapkiewicz@student.uj.edu.pl\n\nhttps://in dico.koza.if.uj.edu.pl/event/7/contributions/743/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/743/ END:VEVENT BEGIN:VEVENT SUMMARY:Angular DOI Calibration Methods towards PET In-System Calibration of (Semi-)Monolithic Scintillators DTSTART;VALUE=DATE-TIME:20220712T083100Z DTEND;VALUE=DATE-TIME:20220712T083300Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-741@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Yannick Kuhl\; RWTH Aachen University\, Germany ()\n Author: Yannick Kuhl\nCo-authors: Florian Mueller\, Stephan Naunheim\, Dav id Schug\, Volkmar Schulz\n\nIntroduction\nAvailable clinical positron emi ssion tomography (PET) systems commonly consist of one-layer segmented det ector arrays with planar 2D gamma positioning. However\, without 3D positi oning in the additional depth-of-interaction (DOI) direction the system’ s spatial resolution is reduced at off-centered positions due to parallax errors. This effect is particularly relevant for small ring diameters\, or for total body PET devices with a large axial field of view. Monolithic a nd semi-monolithic detectors represent an attractive alternative with over all good performance characteristics and intrinsic DOI capability. Current ly\, the best positioning performance of (semi-)monolithic detectors is ac hieved with individual calibration and subsequent processing\, e.g.\, with machine learning-based gamma position estimation routines. Detector calib ration describes the process of acquiring a dataset of gamma interactions in the detector at known positions to generate\, e.g.\, a training dataset for machine learning-based preprocessing approaches. In this work\, the m achine learning technique gradient tree boosting (GTB) is used that establ ishes a relationship between the known gamma interaction position and its light distribution and is thus able to assign an interaction position to u nknown light distributions. However\, the individual calibration is time-c onsuming\, especially for large PET systems\, with calibration times of se veral days per detector using a conventional parallel hole beam collimator . By introducing a fan beam collimator\, also used in this work\, the proc ess can be accelerated to hours per detector and even to minutes with the adaption to a multi fan beam collimator utilizing several fan beams at the same time.\nCurrently\, however\, these methods are only prepared for cal ibration in test setups\, making it tedious to recalibrate the detectors o nce they have been installed in the PET system. A re-calibration may be ne cessary due to changes in measurement parameters\, such as changes in temp erature or bias voltage\, as well as aging of the detector components. The refore\, an in-system calibration technique may be necessary to establish (semi-)monolithic detectors in a system. For planar calibration with gamma irradiation along the detector normal only an adjustment of the setup is necessary. For DOI calibration with currently proven lateral detector irra diation\, however\, a new calibration method must be developed since the d etector sides are inaccessible in a ring.\nThe challenge for in-system cal ibration is now to generate an appropriate DOI training dataset with known irradiation position\, exclusively by measurements in the installed PET s ystem. As a proposed solution\, this work introduces two DOI calibration r outines based on angular detector irradiation.\n\nMaterials\nThe coinciden ce setup consists of a fan beam collimator and two PET detectors based on 8 semi-monolithic LYSO slabs with a height of 19 mm and a width of 3.9 mm coupled to a DPC3200-22 dSiPM (PDPC). One of the detectors is the one to b e evaluated and can be rotated in steps of 11.25° between lateral irradia tion (0°) and the detector normal (90°) in relation to the fan beam plan e by a special bracket. In the set position\, the detector can be driven t hrough the fan beam using a linear translation stage to irradiate the enti re crystal volume for complete calibration datasets.\n\nAngular Irradiatio n Methods\nIn the first “monoAngle” method\, the DOI position of the g amma interactions is obtained from a previously performed planar position estimation\, based on GTB as well\, and the known geometric course of the gamma beam inside the detector.\nIn the second “duoAngle” routine\, th e DOI position is calculated geometrically from the intersection of two su ccessively irradiated beams and then assigned to the gamma interactions wi thin that intersection. The main task here is to find the gamma interactio ns in the area around the intersection. The initial assumption is that the light distributions of both beams are similar at the intersection. An ite rative\, mutual search algorithm narrowing down the light distributions vi a similarity to this intersection showed the best results. In this iterati ve search\, each light distribution of one beam is compared with all gamma interactions of the other beam for similarity using a Nearest Neighbor ro utine with a Euclidean distance metric\, applied to the inverse optical ph oton counts. 20% of the most dissimilar gamma interactions per beam are di scarded per iteration until a termination criterion is reached\, which is based on the Lambert-Beer law and the penetration depth of the beams. Furt her calculated features based on the light distribution as a supplement sh owed no improvement. Using a planar position estimation\, based on GTB and performed in advance\, the gamma interactions in question can be restrict ed to a planar Y-position range around the intersection prior to the itera tive search.\n\nResults and Conclusion\nBoth methods showed a positioning performance within 5 % to the lateral irradiation (0°) up to an irradiati on angle of 45°. Thus\, both methods are suitable candidates for in-syste m calibration. Comparing monoAngle and duoAngle\, the monoAngle method is simpler in application and implementation and could provide even better po sitioning performance for higher resolution detectors than those used here .\n\nhttps://indico.koza.if.uj.edu.pl/event/7/contributions/741/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/741/ END:VEVENT BEGIN:VEVENT SUMMARY:Silicon as a candidate for a proton beam-activated tracer for rang e verification in proton therapy DTSTART;VALUE=DATE-TIME:20220712T082700Z DTEND;VALUE=DATE-TIME:20220712T082900Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-739@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Barbara Kołodziej\; Jagiellonian University\, Polan d ()\nAuthor: Barbara Kołodziej\nCo-authors: Aleksandra Wrońska\, Aleksa ndra Kaszlikowska\, Mareike Profe\, Ronja Hetzel \, Barbara Beus\, Magdale na Garbacz\, Renata Kopeć\n\nProton therapy is a radiotherapy method whic h is superior to conventional radiotherapy performed with photons because of the achievable dose conformality. However\, to fully benefit from the f avorable dose-depth profile of the ion beams\, new methods are required to monitor the treatment process online. A class of methods is under develop ment based on the analysis of prompt gamma radiation\, which can provide i nformation on the dose distribution in real time [1]. The idea of a proton beam-activated tracer\, discussed in this poster\, is based on detecting a prompt gamma signal from an element delivered to the tumor selectively a nd excited by the proton beam [2]. In the poster\, silicon will be present ed as a suitable candidate for such a tracer.\n\nInvestigation on silicon was performed using Geant4 simulations. The aim of the simulations was to obtain a prompt gamma spectrum and the response of a detector. In the simu lations\, a cuboid PMMA phantom was used as the target\, housing in its ce nter a silicone-doped insert (mass concentration of 2%). The phantom was i rradiated using a treatment plan layer by layer\, and the results were adj usted to include the anticipated acceptance of a detector. The use of an H PGe detector of RWTH Aachen [3]\, equipped with an active Compton shield\, was considered. Its response and effect on the registered spectrum were a lso investigated.\n\nFor proximal monoenergetic layers\, the signal from t he tracer was statistically significant when the Bragg peak was completely located in the insert. For distal layers\, when Bragg peak was outside th e insert\, the signal dropped noticeably\, but it was still visible becaus e the cross section for producing gamma particles is non-zero for higher-e nergy protons. Inclusion of the detector response did not change this obse rvation. However\, reduction of statistics resulting from detection effici ency lead to the necessity of including the active Compton shield in the d etection setup\, otherwise the signal was buried by background.\n\nThe res ults showed a dependence between the signal from silicon and the Bragg pea k position\, and this observation can be used to develop monitoring method s based on tracers activated by the proton beam. The next planned step is to confirm this result by experiment\, whose pilot run took place in Febru ary 2022 at the Cyclotron Centre Bronowice. This research was funded by th e Priority Research Area Digiworld under the program Excellence Initiative – Research University at the Jagiellonian University in Kraków and by the Polish National Agency for Academic Exchange.\n\nReferences:\n\nA. Wro ńska i D. Dauvergne. Range verification by means of prompt-gamma detectio n in particle therapy. W: Radiation Detection Systems\, tom 2. CRC Press/R outledge\, 2021. https://doi.org/10.1201/9781003218364-6\nhttps://agenda.i nfn.it/event/23656/contributions/120652/\nLaurent Kelleter et al\, Spectro scopic study of prompt-gamma emission for range verification in proton the rapy\, Physica Medica\, Volume 34\, 2017\, Pages 7-17\, ISSN 1120-1797\, h ttps://doi.org/10.1016/j.ejmp.2017.01.003\n\nhttps://indico.koza.if.uj.edu .pl/event/7/contributions/739/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/739/ END:VEVENT BEGIN:VEVENT SUMMARY:Monte Carlo simulation platform and software stack in Dose-3D proj ect DTSTART;VALUE=DATE-TIME:20220712T081900Z DTEND;VALUE=DATE-TIME:20220712T082100Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-735@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Jakub Hajduga\; AGH University of Science and Techno logy WFIIS\, Poland ()\nAuthors: Jakub Hajduga\, Dose-3D Collaboration\n\n As part of the Dose-3D project titled "Reconfigurable detector for measuri ng spatial distribution of radiation dose for applications in preparing in dividual patient treatment plans"\, in addition to the construction of the new type of phantom itself\, it is necessary to develop high-end software for dose simulation\, configuration and control of the entire device and finally the data analysis. Within the project comprehensiveness the softwa re stack comprises a number of packages for data processing and analysis ( Monte Carlo generators\, Raw data preprocessing\, Machine Learning tools\, Python/C++ modules\, Medical Physics specific software for DICOM standard ).\nThe Monte Carlo simulation itself is an essential element for the succ ess of the Dose-3D project. This is related to the fact that MC physics si mulations are the gold standard cross-check for planning and cross validat ion of experimental data. Using a framework based on the Geant4 engine we will produce data that mimics real apparatus. The data will be used to opt imize the parameters of the Dose-3D cell and ensure proper calibration of the prototype phantom.\nOne of the most important features of the final si mulation platform is the possibility to read-in and setup within simulatio n environment irradiation plans stored in DICOM-RT format.\nOne of the mos t important parts of the Dose-3D software stact is the middleware that bri dges gaps between the low and high level data processing. Based on the Pyt hon-like packages (with specific c++ modules binded to Python) we provide unified services to users. The presented work embraces a modern approach o f software engineering for the interdisciplinary scientific project which is the Dose-3D.\nThe aforementioned middleware in-house software is being developed as Python package called pydose3d. Its modules are responsible f or such functionalities as handling data from different sources (internal MC simulation based on Geant4\, PRIMO simulation and phantom measurements) \, where these data are then unified in a high-level data exchange format (Pandas Data Frame)\, modules responsible for basic visualization and anal ysis of acquired data\, or a set of tools responsible for loading informat ion about the planned course of radiotherapy from DICOM-RT files\, which c an then be loaded into the simulation itself.\n\nhttps://indico.koza.if.uj .edu.pl/event/7/contributions/735/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/735/ END:VEVENT BEGIN:VEVENT SUMMARY:Design of spread-out Bragg peaks in spatially fractionation proton therapy DTSTART;VALUE=DATE-TIME:20220712T082900Z DTEND;VALUE=DATE-TIME:20220712T083100Z DTSTAMP;VALUE=DATE-TIME:20260720T193933Z UID:indico-contribution-33-740@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Agata Tobola-Galus\; Institute Of Nuclear Physics Po lish Academy od Sciences\, Poland ()\nAuthor: Agata Tobola-Galus\nCo-autho rs: Jan Swakoń\, Paweł Olko\nPurpose\n\nIn spatially fractionated proton therapy SFTP (proton grid therapy) the arrays of parallel and pencil prot on beams generated by grid collimator are applied to reduce the impact of irradiation on healthy tissue. At the beam entrance the locally irradiated skin benefits from the ununiform profile of beam causing faster repair of irradiated tissues. In the same time\, due to the multiple Coulomb scatte ring of the proton beam\, the target volume can be uniformly irradiated. I n this paper\, use of proton grid therapy is considered designe Spread-Out Bragg Peak (SOBP) at the target depth. The aim of these studies was dosim etry verification produced grid collimator and range modulator to form uni form SOBP for spatially fractionated proton beams.\nMaterial and Methods\n The experimental verification of the depth dose distribution was performed at the eye proton therapy unit with 60 MeV proton beam from AIC-144 cyclo tron at IFJ PAN Krakow. Mesh-like brass collimators with the lateral centr e-to-centre (c-t-c) spots distances of 2 mm (spot diameter 1 mm were prepa red were prepared. A spread out Bragg peaks (SOBP) of half modulation (fro m 1.5 cm to 3 cm-depth in water) and full modulation were formed by specia lly designed for this collimator range modulators. ProBImS scintillator sy stem with a CCD camera were placed at several depths in a solid-water slab phantom to evaluate the relative 2-D dose distribution. As a figure of me rit\, the ratio between the central dose of one minibeam (peak dose) and t he dose in the middle of two consecutive beams (valley dose) was evaluated . This magnitude\, named peak-to-valley dose ratio (PVDR)\, is a very rele vant parameter in such spatially fractionated techniques. The dosimetry wa s complemented by measurements of the depth dose distribution performed in water phantom using the Markus ionization chamber and 2D-TLD in the solid phantom.\n\nResults\nOur results show that for parallel beams 1 mm in dia meter with c-t-c 2 mm\, the optimal distance of the collimator from the ey elid surface is 35 mm\, obtaining an almost uniform dose in the target and satifiable values of PVDR (3-5) in the region of the beam entrance. A dec rease in the PVDR value is observed with increasing distance of the phanto m from the collimator. Changing the distance of the measurement system fro m the grid collimator (snout) affects the shape of the dose depth distribu tion. The differences are not large\, mainly an increase of the dose at th e entrance\, and negative slope of the plateau are observed.\n\nConclusion s\nSpatially fractionated proton therapy SFPT (proton grid therapy) is bei ng considered for the treatment of eye tumors. A favorable dose distributi on at the beam entry would allow the eye to be irradiated through closed e yelid sparingly due to spatial fractionated dose.\nDesigning range modulat or wheels to SOBP for parallel proton min-beams with energy of 60 MeV\, an d diameter of 1 mm were found to be sufficient achieve homogeneous irradia tion of the target and to obtain a flat plateau SOBP with full and half mo dulation.\nThe results of this work can be used to guide future radiobiolo gical experiments using artificial skin.\n\nThis project has received fund ing from the European Union's H2020 Research and Innovation Programme\, un der Grant Agreement No: 730983\n\nhttps://indico.koza.if.uj.edu.pl/event/7 /contributions/740/ LOCATION:Collegium Maius URL:https://indico.koza.if.uj.edu.pl/event/7/contributions/740/ END:VEVENT END:VCALENDAR