BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:β-lactoglobulin as a platform for designing biologically active c arriers – experimental and computational studies DTSTART;VALUE=DATE-TIME:20211011T134000Z DTEND;VALUE=DATE-TIME:20211011T140000Z DTSTAMP;VALUE=DATE-TIME:20260815T032410Z UID:indico-contribution-22-281@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Paulina Komorek ()\nβ-lactoglobulin (LGB) is known as one of the most interesting transport proteins. In particular\, it can serve as a carrier for hydrophobic molecules\, through the binding of pote ntial ligands at the active site located in the β-barrel [1]. According t o previous studies\, the binding of ligands to LGB is strongly dependent o n the pH of the solution due to the conformational changes of LGB known as the Tanford transition [2]. In the presented study\, the interactions and binding behavior of anesthetic tetracaine (TET) to LGB were investigated under varying environmental conditions (pH\, ionic strength\, concentratio n\, LGB-TET complex molar ratio). The Laser Doppler Velocimetry (LDV)\, th e UV-Vis spectroscopy\, and the Circular Dichroism (CD) were utilized to d etermine the physicochemical properties of LGB and LGB-TET complex in a so dium chloride solution. Electrophoretic mobility measurements showed that the zeta potential of the LGB became more positive upon interactions with TET due to electrostatic forces of the amino group present in the TET stru cture. The finding suggested the formation of LGB-TET complexes and the bi nding of ligand molecules on the protein surface. Based on UV-vis spectra the binding constant (K-UV) of the LGB-TET complex was calculated\, while CD spectra showed that interactions with the ligand did not change the sec ondary structure of LGB molecules. Quartz Crystal Microbalance with Dissip ation Monitoring (QCM-D) measurements presented that the molar ratio of LG B to TET is equal to 1:13 confirming the binding of TET not only to β-bar rel but also on the LGB surface. What is more\, QCM-D performed under vary ing environmental conditions allowed determining the optimized conditions for LGB-TET complex formation. Implementation of molecular docking enabled estimation of the binding position of the TET. The method suggested that interactions between the protein and ligand were possible with the most li kely binding site with the hydrophobic cavity located in β-barrel [3].\n* *References:** \n1. G. Kontopidis\, C. Holt\, L. Sawyer. Invited Review: β-Lactoglobulin: Binding Properties\, Structure\, and Function. Journal o f Dairy Science 2004\, 87(4)\, 785-796\n2. B. Y. Qin\, M. C. Bewley\, L. K . Creamer\, H. M. Baker\, E. N. Baker\, G. B. Jameson. Structural basis of the Tanford transition of bovine beta-lactoglobulin. Biochemistry 1998\, 37(40)\, 14014-14023\n3. S. Świątek\, P. Komorek\, G. Turner\, B. Jachim ska. β-Lactoglobulin as a potential carrier for bioactive molecules. Bioe lectrochemistry 2019\, 126\, 137-145\n**Acknowledgments:**\nThis work was partially supported by project NCN OPUS 2016/23/B/ST5/02788 and InterDokMe d POWR.03.02.00-00-I013/16.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/c ontributions/281/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/281/ END:VEVENT BEGIN:VEVENT SUMMARY:Total-Body PET: System Design and Applications DTSTART;VALUE=DATE-TIME:20211011T142000Z DTEND;VALUE=DATE-TIME:20211011T145000Z DTSTAMP;VALUE=DATE-TIME:20260815T032410Z UID:indico-contribution-22-279@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Suleman Surti (University of Pennsylvania)\nThe curr ent generation of commercial PET scanners has excellent performance and di agnostic image quality\, but the system sensitivity and dynamic imaging ca pability are limited by the scanner’s axial length. In recent years ther e has been an interest in developing whole-body PET scanners with much lon ger AFOV that not only increase the system sensitivity but can also image the whole-body of a patient without bed translation. Currently there are a t least two commercial scanners offering at least 1 m long axial field-of- view (AFOV). An important outcome of very high sensitivity is the potentia l to significantly reduce routine clinical scan times which can be benefic ial in reducing patient motion artifacts and increase patient throughput. Alternately\, the injected dose can be reduced that is beneficial in areas such as pediatric imaging and serial imaging of patients for monitoring r esponse to therapy. Whole-body imaging with large axial coverage will allo w one to perform dynamic imaging for pharmacokinetic studies over multiple organs. In this presentation we will present the design concepts underlyi ng the development of long AFOV systems (two commercial and one research)\ , followed by a few example studies illustrating the imaging capabilities and clinical/research potential of such systems. Finally\, new design conc epts that aim to reduce the cost of these system will be introduced\n\nhtt ps://indico.koza.if.uj.edu.pl/event/4/contributions/279/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/279/ END:VEVENT BEGIN:VEVENT SUMMARY:Positronium Imaging with the J-PET detector for the medical purpos es DTSTART;VALUE=DATE-TIME:20211011T140000Z DTEND;VALUE=DATE-TIME:20211011T142000Z DTSTAMP;VALUE=DATE-TIME:20260815T032410Z UID:indico-contribution-22-260@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kamil Dulski (Jagiellonian University)\nPositronium Imaging [1-4] is a branch of Positron Emission Tomography (PET) which focu ses on the spatial and structural correlation probed by positronium (posit ron-electron atom) formation in the test sample or in the tissues of patie nt. It is possible due to the (main) influence of the size of the free vol umes (nm scale) on the mean lifetime of the long-lived positronium state - ortho-positronium (total spin number S = 1) [1-6]. Moreover\, the positio n of positronium decay can be reconstructed based on its decay products – high-energy photons [1-8]. Therefore\, by using additional marker of t he positronium formation the lifetimes and the positions of a single decay s of positronia can be collected during a scan. The positronium images con sist of an estimate of the mean positronium lifetime in each image voxel. Such marker that can be used to estimate the formation of the positronium is an additional photon associated with the creation of a positron which f orms positronium with an electron from the tested sample [1-4]. Due to the additional contrast that can differentiate healthy cells from neoplastic cells from the mean lifetime of the positronium\, the potential of PET to detect neoplastic lesions may be increased\, where the possibilities of de termining the degree of malignancy from positronium imaging are also discu ssed [1-4]. The first demonstration of positronium imaging was performed o n the J-PET detector [1]\, which is based on an innovative technology that benefits from the excellent timing capabilities of plastic scintillators (resolution ≈ 100 ps) as well as a relatively large axial field-of-view (FOV ≈ 0.5 m) [3\, 5-8]. Fundamentals of positronium imaging and results from the imaging of the phantom consisting of heart tumor tissues (Cardia c Myxoma) and normal pericardial tissues by the J-PET detector will be sho wn. These are also the first images obtained by positronium imaging\, and the J-PET detector is the first device capable of collecting such images.\ nReferences:\n[1] P. Moskal\, K. Dulski et al.\, Science Advances 2021 (in press)\n[2] P. Moskal … K. Dulski et al.\, EJNMMI Phys. 7 (2020) 44\n[3 ] P. Moskal and E.Ł. Stępień\, PET Clin. 15 (2020) 439\n[4] P. Moskal e t al.\, Nature Rev. Phys. 1 (2019) 527\n[5] K. Dulski et al.\, NIM A 1008 (2021) 165452\n[6] K. Dulski et al.\, Hyperfine Interact. 239 (2018) 40\n[ 7] P. Moskal … K. Dulski et al.\, IEEE Trans. Instrum. Meas. 70 (2021) 2 000810\n[8] S. Niedżwiecki … K. Dulski et al.\, Acta Phys. Pol. B 48 (2 017) 1567\nAcknowledgment:\nThis work was supported by the Foundation for Polish Science (FNP) through grant TEAM/2017- 4/39\, nom. N17/MNS/000023 a nd the DigiWorld (Total-Body Jagiellonian-PET Laboratory) and the SciMat ( PSP: U1U/P05/NW/03.23) Priority Research Area as part of the Excellence In itiative program at the Jagiellonian University.\n\nhttps://indico.koza.if .uj.edu.pl/event/4/contributions/260/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/260/ END:VEVENT BEGIN:VEVENT SUMMARY:Uncovering the diagnostic power of exosomes for prosthetic joint f ailure DTSTART;VALUE=DATE-TIME:20211011T132000Z DTEND;VALUE=DATE-TIME:20211011T134000Z DTSTAMP;VALUE=DATE-TIME:20260815T032410Z UID:indico-contribution-22-244@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Ana Ribeiro (International Iberian Nanotechnology La boratory)\nThe effect of debris exposure on the osteoimmunological crossta lk is poorly understood. For the first time\, we report that titanium diox ide nanoparticles (TiO2 NPs)\, similar in size and composition to wear deb ris associated with prosthetic implants\, altered bone exosomes biogenesis and cargo. Using mass spectrometry analysis\, we identified urokinase-typ e plasminogen activator (uPA)\, specifically enriched in exosomes derived from bone cells pre-incubated with TiO2 NPs. Besides uPA contribution to t he generation of inflammatory signals\, uPA was also previously reported i n patients with aseptic loosening of total hip prosthesis. Functional test s with isolated bone derived exosomes confirmed the activation of human ma crophages with consequent secretion of inflammatory cytokines that may con tribute to particle induced osteolysis and implant loosening. These findin gs\, indicate that the osteoimmunological communication trough exosomes wa s disturbed by TiO2 NPs and that uPA may be proposed as a biomarker to ear ly diagnose nanoparticle induced osteolysis\, avoiding or delaying a revis ion surgery\, thereby decreasing disease burden and improving patient heal th.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions/244/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/244/ END:VEVENT BEGIN:VEVENT SUMMARY:Targeted nanoparticles for cancer detection in animal models. DTSTART;VALUE=DATE-TIME:20211011T130000Z DTEND;VALUE=DATE-TIME:20211011T132000Z DTSTAMP;VALUE=DATE-TIME:20260815T032410Z UID:indico-contribution-22-233@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Barbara Błasiak (Polish Academy of Sciences\, Insti tute of Nuclear Physics\; Department of Clinical Neurosciences\, Universi ty of Calgary)\nMagnetic Resonance Imaging (MRI) has been used for early c ancer detection\, as it provides high spatial resolution and soft tissue c ontrast. Yet its specificity is low. Standard contrast enhanced MRI is bas ed on tumors vasculature (i.e. Gd-based) and it does not provide sufficien tly high specificity for tumor diagnosis and thus targeted contrast agents providing T2 contrast have been applied to provide information on tumor s pecificity[1\,2]. \nTherefore\, we have developed core/shell NaDyF4/NaGdF4 nanoparticles changing both T1 and T2 relaxation times of surrounding wat er molecules. The NPs were conjugated with tumor specific antibodies and p roteins. The relaxation times (T1 and T2) of the nanoparticles with variou s core/shell sizes and concentrations were measured at 9.4T and 3T to find the optimum T1/T2 ratio for maximum contrast. T1- and T2-weighted images using core/shell nanoparticles of the animal models of brain\, breast and prostate cancer were collected. Mouse models of cancer were used at 9.4T. We imaged 6 weeks nude mice with the tumor before the injection of the tar geted and non-targeted contrast agents and in different time after injecti on (10 min after\,1h\, 2h and 24h). The core/shell based NPs provided impr oved tumor contrast when the T1 and T2-weighted MR pulse sequences were ap plied. The results show that the developed NPs may improve the efficacy of MRI in cancer detection.\nReferences:\n1. Blasiak B\, Tomanek B\, et al D etection of T2 changes in an early mouse brain tumor. Mag Res Imag 2010\, 28:784-9.\n2. Tomanek B\, Iqbal U\, Blasiak B\, at al. Evaluation of Brain Tumor Vessels Specific Contrast Agents for Glioblastoma Imaging. Neuro-On cology\, 2012\, 14(1):53-63.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/c ontributions/233/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/233/ END:VEVENT END:VCALENDAR