BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:A new model for spheroid growth DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-307@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Monika Szczepanek (Department of Medical Physics\, M . Smoluchowski Institute of Physics\, Faculty of Physics\, Astronomy and A pplied Computer Science\, Jagiellonian University\, Kraków\, Poland\, Tot al-Body Jagiellonian-PET Laboratory\, Jagiellonian University\, Poland)\nS pheroids are a model commonly used in research into new cancer treatments and therapies. They mimic the structure\, microenvironment and cells signa ling present in solid tumors [1]. The following features can be recalled\, that makes spheroids a perfect model for investigations into cancer treat ment\, like their layered composition\, forced by the availability of nutr ients\, the growth kinetics and the expression pattern of some genes simil ar in spheroids and tumors [2\,3]. In particular\, three types of cells in the spheroid\, as well as in tumors\, can be distinguished\, which mainta in their layered architecture: dead cells inside the spheroid (necrotic zo ne)\; living\, non-proliferating cells in the middle (quiescent zone)\; an d living and proliferating cells in the outer layer of the spheroid [4\,5] . We propose a new spheroid growth model which reveals the growth dynamics of three spheroid zones. The model assumes different probabilities of the cell transition from proliferating to non-proliferating cells and from no n-proliferating to necrotic ones. This biological process goes only in one direction. We present a theoretical model based on simulations and experi mental data. By the presented model\, it is possible to assess proliferati ve and non proliferating cells\, which may be helpful in an experiment pla nning\, when particular fraction of proliferating cells are needed. In add ition\, the simulation data allow not only to confirm the prediction from the model used\, but also to check how cells in a given state are distribu ted inside the spheroids. In particular\, the model allows an additional e stimation of the fraction of dead cells indirectly\, only from the growth curve. Therefore\, the presented model can potentially provide more inform ation than the standard approach in such studies of the growth dynamics of tumors – the Gompertz curve.\n**References:**\n[1] A. S. Nunes et al.\, Biotechnol. Bioeng. 116\, (2019).\n[2] E.Ł. Stępień et al.\, Acta Phys . Pol. B 51\, (2020).\n[3] E. Axpe et al.\, PLoS One 9\, (2014).\n[4] M. S zczepanek\, Acta Phys. Pol. B 51\, (2020).\n[5] H. Karimi et al\, Micron 1 37\, (2020).\n**Acknowledgments:**\nThis work was supported by the Foundat ion for Polish Science through the TEAM POIR.04.04.00-00-4204/17 Programme and the SciMat Priority Research Area budget under the Strategic Programm e Excellence Initiative at the Jagiellonian University through grant No. U 1U/P05/NW/03.23.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions /307/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/307/ END:VEVENT BEGIN:VEVENT SUMMARY:ATR-FTIR spectroscopy of extracellular vesicles derived from endot helial cells cultured in hyperglycemic conditions DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-303@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Carina Rząca (Jagiellonian University)\ncarina.rzac a@doctoral.uj.edu.pl\n\nNowadays extracellular vesicles (EVs) are being ac tively researched. EVs are involved with several biological processes incl uding cell signalling\, transfer specific cargo (lipids\, proteins\, and n ucleic acids) and biomarkers of disease. EVs can be divided according to t heir size and way of arising into exosomes (diameter from 30 nm to 100 nm) and ectosomes (diameter from 100 nm to 1000 nm) [1\,2\,3].\n\nThe Attenua ted Total Reflectance Fourier Transform Infrared spectroscopy (ATR-FTIR) m ethod is based on the characteristic absorption of infrared radiation at s pecific wavelengths by functional groups like N–H\, C=O\, CH2\, CH3\, an d PO2. An IR spectrum carries specific information on the sample’s molec ular composition and structure [6\,7\,8]. The aim of this study is investi gation endothelial EVs cargo modifications in hyperglycemic conditions.\n\ nIn this experiment we used cells\, exosomes and ectosomes derived from te lomerase-immortalized human microvascular endothelium cell line (TIME) cul tured in normoglycemic and hyperglycemic conditions. The parameters were d etermined to characterize the chemical state of the lipids and proteins of the EVs: saturated to unsaturated fat ratio\, acyl chain length\, protein phosphorylation and lipid to protein ratio [2\,6]. In addition\, the perc entage contribution of the following secondary protein structures was calc ulated based on the analysis of the second derivative of the spectra in th e Amide I band range: side chain\, inter β-sheet\, β-sheet\, random coil \, α-helix and β-turn [2].\n\nFTIR results showed that exosomes\, ectoso mes and cells differ in content of protein and lipid components. Moreover\ , obtained results revealed differences in the molecular composition and s econdary structures of proteins from EV subpopulations derived from hyperg lycemic endothelial cells. Statistically significant differences were foun d between ectosomes from normoglycemia and hyperglycemia conditions for th e values of almost all calculated parameters. Summarizing\, ectosomes can be considered as diabetes biomarkers. ATR-FTIR analyses may be useful in i dentifying new biomarkers of diabetes and its complications.\n\nReferences \n1. Stępień EŁ et al. Arch. Med. Res. 2012\;43: 31-35.\n2. Stępień E Ł et al. Biochem Biophys Rep. 2021\;25:100888.\n3. Roman M et al. Nanomed icine. 2019\;17:137-149.\n4. Stępień EŁ et. al. Theranostics. 2018\;8:3 874-3890.\n5. Alexandru N et al. Biochem Biophys Res Commun. 2016\;472:1-1 0.\n6. Ricciardi V et al. Appl. Sci. 2020\;10:2974.\n7. Dogan A et al. Gen omics 2013\, 14:386\n8. Kumar S et al. Chem. Soc. Rev.\, 2016\, 45\, 1879. \n\nAcknowledgments \nThis study was supported by the Polish NCN grant OPU S 17 to prof. E. Stępień (2019/33/B/NZ3/01004) and the SciMat Priority R esearch Area budget under the Strategic Programme Excellence Initiative at the UJ.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions/303/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/303/ END:VEVENT BEGIN:VEVENT SUMMARY:Assessment of the influence of the Beta parameter in the reconstru ction of Q.Clear. DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-296@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Konrad Skórkiewicz (Jagiellonian Univeristy)\nMolec ular PET / CT imaging is used for the diagnosis of patients with neuroendo crine tumors with the use of radiolabelled somatostatin analogues. Iterati ve image reconstruction techniques are used to obtain the image.Unfortunat ely\, as a result of the significant impact of the so-called "Partial Volu me Effect" in minor changes in pathological radiopharmaceutical uptake\, t he results of the quantitative assessment are underestimated. In PET image s\, it affects the assessment of the diagnostic test results as a false ne gative result. The selection of appropriate Q.Clear reconstruction paramet ers in the PET / CT MI DR system can reduce the impact of this phenomenon. In order to perform the appropriate analysis\, measurements has been made using the NEMA IEC PET Body Phantom\, in which the hot spheres have been filled concentration 10:1 of 68Ga isotope. The raw data was reconstructed using a Q.Clear reconstruction for a Beta parameter in the range 150-1000\ , in steps of 50. The analysis showed a clear decrease in the maximum valu es and mean SUVs with higher values of the Beta parameter for the smallest spheres.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions/296/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/296/ END:VEVENT BEGIN:VEVENT SUMMARY:Myocardial perfusion scintigraphy - criteria of SPECT/CT protocol selection. DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-297@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Adrian Kania-Kuc (Nuclear Medicine Unit\, Department of Endocrinology Department of Endocrinology\, Oncological Endocrinology and Nuclear Medicine\, University Hospital\, Kraków\, Poland)\n**Key word s:**\nmyocardial perfusion\, nuclear medicine\, SPECT/CT\, image quality\n \n**Background:**\nMyocardial perfusion imaging with 99mTc-MIBI by SPECT/C T plays a major role in the diagnosis of coronary artery disease (CAD) as a non-invasive test to assess perfusion in cardiomyocytes. The exam allows to assess the severity of the disease\, the effectiveness of the therapy\ , and has a prognostic value. One disadvantage of this imaging method is t he relatively long SPECT acquisition time. The possible solution to this p roblem is shortening of the examination time by improving the quality of imaging and changing the acquisition parameters. The use of SPECT/CT with cadmium-zinc telluride (CZT) technology may improve scanning parameters in cluding reduction of acquisition time\, however maintenance of high-qualit y imaging is required.\n\n**Aim:**\nThe aim of the study was to check the possibility of implementation of a new myocardial perfusion imaging protoc ol with reduced acquisition time.\n\n**Material and methods:**\nWe compare d two protocols of myocardial perfusion SPECT/CT with 99mTc-sestamibi usin g different acquisition and reconstruction parameters. Two scans for one p atient was done firstly as an 8 minutes protocol\, the second was shortene d to 6 minutes. The acquisition was performed on a CZT camera one after th e other. Then CT scan for attenuation correction was performed according t o the standard SPECT/CT procedure. The analysis was performed on planar pe rfusion reconstruction of the SPECT myocardium. The volume of the greatest distribution of radionuclides in the heart was selected semi-automaticall y on the obtained coronary images. For both protocols\, three measurements were performed at the same locations and similar volumes in each measurem ent\, calculating the number of counts\, mean\, standard deviation (SD) an d volume. The measured volumes gradually increased in the measurements. Th e SD standardized to the volume of 1 ml was considered an important parame ter of the differentiation capacity of regions with a lower uptake of 99mT c-sestamibi in cardiomyocytes. All images were assessed by two nuclear med icine specialists in order to confirm the quality of the imaging.\n\n**Res ults:**\nPerformed examination of one patient with confirmed myocardial is chemia indicated a 10.48% decrease in SD/ml and 23.62% decrease in the num ber of counts of the measured volume. The amount of decrease in SD/ml sign ificantly reduced the possibility of diagnosis of small ischemia regions i n 6 minutes protocol in comparison with 8 minutes protocol.\n\n**Conclusio n:**\nOn the basis of comparative analysis performed by nuclear medicine s pecialists\, the 8-minute protocol was selected as the standard SPECT/CT m yocardial perfusion procedure due to better image quality and greater reso lution for small ischemic area of myocardium.\n\nhttps://indico.koza.if.uj .edu.pl/event/4/contributions/297/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/297/ END:VEVENT BEGIN:VEVENT SUMMARY:Optimization and enhancement of CNR in MRI using core/shell contra st agent DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-256@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: David MacDonald ()\nTitle:\nOptimization and enhance ment of CNR in MRI using core/shell contrast agent\n\nD. MacDonald [1]\, B . Blasiak [1] [2]\, B. Tomanek [1] [2] [3]\n\n\nPresenting author:\nDavid MacDonald\ndmlmacdona@gmail.com\n\n\nMagnetic resonance imaging (MRI) prov ides the best soft contrast tissue among diagnostic imaging modalities suc h as CT\, PET or X-ray. The contrast provided by MRI is based on the proto n density and on interactions of protons with the surrounding molecules of tissues causing so called T_1 and T_2 relaxations. MRI techniques utiliz e these processes for contrast manipulation by producing T_1 or T_2 weig hted MR images. While MRI contrast may be provided solely by tissues thems elves due to differences in their relaxation times\, contrast agents short ening T_1 and T_2 further improve detection of small pathologies such as early stages breast or brain cancers. Recently T_1/T_2 core shell contrast agents have been developed with an expectation that the contrast to noise ratio (CNR) would be greater than when compared to T_2 contrast agent. T o prove this\, firstly we calculated optimal parameters in commonly used S pin Echo and IR TrueFISP pulse sequences that provide the greatest CNR for known T_1 and T_2 relaxation times for an animal model of breast cancer. The results show that the CNR of a tumor for a T_1/T_2 core shell contras t agent is greater than that of just a T_2 contrast agent for both the Sp in Echo and IR TrueFISP pulse sequences. To demonstrate the potential of o ur core/shell contrast agent in vivo MRI we imaged mice with breast tumors after intravenous injection of 0.25 mL of non-targeted core shell contras t agent NaDyF4 (20 nm)/NaGdF4 (~ 0.5 nm). Then to further increase the CNR \, we subtracted a T_1 weighted image with T_2 weighted image. Post-inje ction results show that the best CNR comes from the T_1 weighted image sub tracted by the T_2 weighted image\, and the CNR for the T_1 weighted imag e is greater than the CNR for the T_2 weighted image.\n\n[1] Instytut Fizy ki Jadrowej Polish Academy of Science\, Walerego Eljasza Radzikowskiego 15 2\, 31-342 Kraków\, Poland\n[2] University of Alberta\, 116 St & 85 Ave\, Edmonton\, AB T6G 2R3\, Canada\n[3] University of Calgary\, 2500 Universi ty Dr NW\, Calgary\, AB T2N 1N4\, Canada\n\nhttps://indico.koza.if.uj.edu. pl/event/4/contributions/256/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/256/ END:VEVENT BEGIN:VEVENT SUMMARY:A simulation study to compare performance of analog and digital si licon photomultiplier tube by LTspice package DTSTART;VALUE=DATE-TIME:20211010T130000Z DTEND;VALUE=DATE-TIME:20211010T132000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-231@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Keyvan Tayefi Ardebili ()\nDetectors play a fundamen tal role in understanding physical phenomena and improved our understandin g. The rapid development in this area of science has been increased intere st to enhance the performance of detectors to achieve precise results. Pho tomultiplier tube (PMT) known as one of the main parts of the detectors wh ich are constructed in the shape of a vacuum tube and can detect light pho tons emitted by scintillators and amplify the intensity of light up to 100 times. Alongside the advantages of PMT\, it has weaknesses such as being sensitive to a magnetic field which prevents their use in magnetic resonan ce imaging (MRI)\, low gain\, and less coverage of scintillators. These we aknesses caused to development of an alternative type of element called si licon photomultipliers (SiPM). SiPMs are the latest generation of photomul tipliers\, which due to their low operating voltage\, are currently used b y many groups in a variety of fields\, including high-energy physics calor imetry\, solid-state physics\, and nuclear medicine. SiPMs consist of inde pendent pixels that are connected parallel to each other. It has a rectang ular sensitive cross-section where each pixel consists of a series connect ion of an avalanche photodiode (APD) in Geiger mode and a quenching resist or. Thanks to this geometrical design\, SiPM are able to provide larger se nsitive areas in comparison to PMTs. In this study\, we made a comparison of analog and digital SiPM as the most popular and recent type of photomul tipliers via LTspice simulation package. The obtained results can provide a general perspective for each type for further utilization.\nReferences:\ n[1] S. Yamamoto\, et al.\, Med. Phys.\, vol. 39\, 2012\, pp. 6900-6907.\n [2] B.K. Lubsandorzhiev\, Nucl. Inst. Meth. in Phys. Res. Sec. A\, vol. 5 67\, 2006\, pp. 236-238\n[3] S. Gundacker\, A. Heering\, Phys. Med. Biol. vol. 65\, 2020\, pp. 101-108. \n[4] F. Acerbia\, S. Gundacker\, Nucl. Inst . Meth. in Phys. Res. Sec. A\, vol. 926\, 2006\, pp. 16-35.\n[5] I. Bokat i\, V. Korotaev\, et al.\, Phot. Mat. A\, vol. 11367\, 2020\, pp. 78-83. \ n[6] P. Lecoq\, S. Gundacker\, Eue. Phys\, J\, vol. 136\, 2021\, pp. 213-2 21.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions/231/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/231/ END:VEVENT BEGIN:VEVENT SUMMARY:The Present and the future of Breast Cancer diagnosis DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-263@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Shivani . (Jagiellonian University)\nThe purpose of the presented investigation is to compare the sensitivity\, specificity\, PPV\, and NPV for standard mammography\, spectral mammography\, ultrasound \, and magnetic resource imaging (MRI)\, which are the commercially availa ble imaging modalities for breast cancer. The aim of our investigation is to design\, construct and establish the performance characteristics of the Jagiellonian Positron Emission Mammography (J-PEM)\, for the detection an d diagnosis of cancer. Its construction is based on a novel idea of PET to mography based on plastic scintillators [1\,2] and wavelength shifters (WL S) [7\,8] and a new concept of positronium imaging [3\,4\,5]. This study c haracterizes the performance of a newly developed J-PEM scanner prototype. The prototype system consists of a single module of plastic scintillators \, built from two layers of the plastic scintillator (6x24x500 mm) and one layer of the wavelength shifters (3x10x100 mm) [6\,7] placed orthogonally between them. Each scintillator bar is attached at both ends to Silicon P hotomultipliers for the signal readout. This 3D system is based on the nov el idea of applying plastic scintillators to detect annihilation photons a nd improving spatial resolution by utilization of wavelength shifters (WLS ). J-PEM can be an effective system for the detection and diagnosis of bre ast cancer in its early stage by improving sensitivity and specificity and it can be achieved by the combined use of plastic scintillators\, which h ave superior timing properties\, with the WLS. In addition\, this device w ill be developed in view of the classification of malignancy based on the possibility of positronium mean lifetime imaging.\n**References**: \n[1] P . Moskal\, Sz. Niedźwiecki\, et al.\, "Test of a single module of the J-P ET scanner based on plastic scintillators\," Nucl. Instr. Meth. A 764\, 31 7 (2014).\n[2] P. Moskal\, O. Rundel\, et al.\, "Time resolution of the pl astic scintillator strips with matrix photomultiplier readout for J-PET to mograph\," Phys. Med. Biol. 61\, 2025 (2016).\n[3] P. Moskal\, D. Kisielew ska\, et al.\, "Feasibility study of the positronium imaging with the J-PE T tomograph\," Phys. Med. Biol. 64\, 055017 (2019).\n[4] P. Moskal\, B. Ja sińska\, et al.\, "Positronium in medicine and biology\," Nature Reviews Physics 1\, 527-529 (2019).\n[5] P. Moskal\, D. Kisielewska\, et al.\, "Pe rformance assessment of the 2gamma positronium imaging with the total-body PET scanners\, "EJNMMI Physics. 7:44 (2020).\n[6] J. Smyrski\, P. Moskal\ , et al.\, "Application of WLS strips for position determination in Strip PET tomograph based on plastic scintillator\, "BioAlgorithms and Med-Syste ms 10\, 59 (2014).\n[7] J. Smyrski\, et al.\, "Measurement of gamma quantu m interaction point in plastic scintillator with WLS strips\," Nuclear Ins t. and Methods in Physics Research A 851\, 39-42\, (2017).\n\nhttps://indi co.koza.if.uj.edu.pl/event/4/contributions/263/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/263/ END:VEVENT BEGIN:VEVENT SUMMARY:Classification of heavy metal contaminated samples based on micro- CT images using machine learning algorithms DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-258@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Dominik Panek (M. Smoluchowski Institute of Physics\ , Jagiellonian University)\nBeing able to predict whether a sample is cont aminated with any toxic substances or not is crucial\, especially when it comes to health\, and this is where machine learning algorithms are essent ial [1-2]. In our research the emphasis was put on the ability to classify samples contaminated with two types of heavy metals: zinc (Zn)\, cadmium (Cd)\, and the mixture of these two elements (Zn+Cd)\, based on the micro- computed tomography (micro-CT) images. Contaminated samples\, which were o perculum\, were coming from the Carassius Gibelio fish. Prior to the micro -CT scan fish were bred in the environment containing a concentration of 4 mg/ml of water of each element. Additionally\, a control group\, with no exposure to any heavy metal was cultured.\nAfter micro-CT scans\, images w ere reconstructed in order to get information\, which would help algorithm s learn about the dataset\, and finally\, which would be able to classify samples into proper groups. The key features of the reconstructed images w ere: grayscale maximum value in a given group\, masses of the samples\, me an grayscale values\, and area under the grayscale histograms. \nApplied m achine learning models included: logistic regression\, SVM (Supporting Vec tor Machine)\, decision trees\, and KNN (K-nearest neighbors\, with differ ent numbers of neighbors k = 1\, 2\, 3\, …\, 10). Results left us no dou bt that most of the applied machine learning models are very good when it comes to classification. The best results were achieved for the simplest l ogistic regression\, where the overall accuracy was 90%\, a second-best al gorithm was KNN with an accuracy of 71% for k = 1 and 86% for k = 4\, next were decision trees with an accuracy of 70% and SVM with an overall accur acy of 50%.\n\nReferences\n[1] Heavy metals in suspended matters during a tidal cycle in the turbidity maximum around the Yangtze Estuary\, Huaijing Zhang et al.\, Acta Oceanologica Sinica 34\, 36–45(2015).\n[2] Toxicity \, mechanism and health effects of some heavy metals\, Monisha Jaishankar\ , Interdiscip Toxicol. 2014 Jun\; 7(2): 60–72.\n\nhttps://indico.koza.if .uj.edu.pl/event/4/contributions/258/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/258/ END:VEVENT BEGIN:VEVENT SUMMARY:BSA as a biologically active nanocarriers – computational studie s DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-289@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Kamil Rakowski (Jerzy Haber Instiute of Catalysis a nd Surface Chemistry Polish Academy of Sciences )\nHuman albumin (HSA) is the main plasma protein that ensures the maintenance of proper osmotic blo od pressure and is also involved in the transport of metabolites to cells. It exhibits high solubility at pH = 7.4 and the ability to bind molecules \, making it possible to use it as a transporter of drugs such as 5-fluoro uracil (5-FU). 5-FU is a drug that causes the incorporation of fluoronucle otides in place of nucleotides that inhibit the thymidylate synthesis of t he nucleic acid enzyme. 5-Fluorouracil is used to treat a number of cancer s. The biggest problem is its susceptibility to dihydropyrimidine dehydrog enase (DPD)\, which metabolizes 5FU to the form of dihydrofluorouracil (DH FU) and destroys its therapeutic activity. The crystallographic model of b ovine albumin (BSA)\, which is an equivalent of human albumin (HSA)\, was selected for the research. Before the docking process\, the model was prep ared in the Gromacs program. The object was simulated until the conformati onal changes stabilized\, which was monitored via the RMSD function. Final ly\, the thus obtained BSA model was used for interaction with 5-FU. Befor e the docking process\, the drug was prepared in the Avogadro program. Hyd rogens corresponding to a protonation state of pH = 7.4 were added to the drug molecule and minimized in the MMFF94 field using a Conjugate Gradient . The standard protocol of random docking on the whole protein volume was used\, the MGLTools tool was used\, docked using the rigid Autodock Vin me thods. The lowest energy complex was simulated with MD\, the drug-free con trol and the protein/ligand complex were simulated for another 100ns under the same conditions. In the conducted research\, the ligand was randomly docked in the entire BSA volume. The results unique for the protein were v isualized\, and the complex with the most favorable energy was simulated u sing molecular dynamics methods. BSA has been shown to bind 5-FU at a simi lar position as HSA in the IB domain. Moreover\, it has been established b y computational methods that the binding of 5-FU at the center of the prot ein (IIIA and IIA domains) may be the most common and energetically most b eneficial for BSA. Docking at the center with the lowest Gibbs free energy was investigated in detail. Hydrophobic domains inside the ligand-binding pocket have been shown to influence the organization of solvent molecules and the formation of water clusters. The formed clusters constitute the m ain mechanism that stabilizes the drug inside the canal\, which may show p romise for its controlled release at elevated temperatures. During the sim ulation\, 5-FU moves into the cavity between domains IIIA and IIA. The bin ding of 5-FU may affect the mobility of adjacent BSA domains\, in particul ar the IB domain\, which is one of the most important pockets for binding substances with therapeutic potential. The presence of the ligand between domains IIIA and IIA resulting in the appearance of strong local chain flu ctuations (110-118AA\, IB domain).\n\nhttps://indico.koza.if.uj.edu.pl/eve nt/4/contributions/289/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/289/ END:VEVENT BEGIN:VEVENT SUMMARY:The development of a method for determining ortho-Positronium mean lifetime in extracellular vesicles using Positron Annihilation Lifetime S pectroscopy DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-285@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Julia Nizioł (Faculty of Physics\, Astronomy and Ap plied Computer Science\, Jagiellonian University\, Kraków\, Poland)\nPosi tron annihilation lifetime spectroscopy (PALS) has been used less extensiv ely in studies with biological material\, although its use in interrogatin g free volume\, voids and defects in polymers is well established. There e xist a number of results\, e. g. by E. Kubicz and the J-PET group [1]\, s howing the correlation between cell structure and the PALS parameters\, su ch as mean ortho-Positronium (o-Ps) lifetime and intensity. This technique has also been demonstrated to have utility as an in situ molecular probe in self-assembled biomimetic systems for which it is highly sensitive to c onformational\, structural and microenvironmental transformations [2]. \n\ nExtracellular vesicles (EVs) are defined as bilayer cell membrane fragmen ts released into the extracellular space by various types of cells. EVs pl ay dual role throughout the body\, due to their involvement in both physio logical and pathological conditions [3]. Growing interest in these spheric al structures emerges from their involvement in cell-to-cell communication \, tumour progression and their possible application as biomarkers or drug delivery systems [4]. \n\nApplying PALS to study EVs required set-up mo difications (chamber design) and calibrations\, in order to adjust the sys tem for studies of liquid samples in temperature controlled conditions. Fo r that purpose\, the system was equipped with the Lauda LOOP L100 thermost at. Temperature of investigated samples was estimated from calibration dat a obtained through extensive thermal testing.\n Two EV samples derived fro m normal pancreatic beta-cell cultures suspended in PBS solution were exam ined: (1) from culture under normoglycemic and (2) hyperglycemic condition s. EV concentrations in the samples were determined using qNano technique and its values were respectively: (1) $9\\times10^{10}$ and (2) $6\,9\\tim es10^{10}$ particles/mL. \n\n Preliminary results demonstrate strong corre lation between mean o-Ps lifetime and EV concentration in the sample. Stud ied concentrations of EVs were too low\, therefore it was mainly the PBS s olution that was contributing to the resulting o-Ps lifetime value\, and n ot the EVs itself. \n Obtained result opens perspective for further resear ch\, when applying higher EVs to PBS ratio. Such experiments were performe d e. g. by P. Sane et al. [5] and demonstrated that observing changes in o -Ps lifetime\, corresponding to phase transitions of membrane lipids in ve sicles (multilamellar DPPC)\, is feasible with PALS technique.\n\nReferenc es:\n[1] Kubicz\, E.\, Doctoral Thesis\, Jagiellonian University (2020).\n [2] Fong\, C. et al.\, Physical chemistry chemical physics : PCCP vol. 17\ ,27: 17527-40 (2015).\n[3] Stępień\, E. et al.\, Expert opinion on thera peutic targets vol. 16\,7: 677-88 (2012).\n[4] Surman\, M. et al.\, Curren t pharmaceutical design vol. 25\,2: 132-154 (2019).\n[5] Sane\, P. et al.\ , The journal of physical chemistry. B vol. 113\,7: 1810-2 (2009).\n\nhttp s://indico.koza.if.uj.edu.pl/event/4/contributions/285/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/285/ END:VEVENT BEGIN:VEVENT SUMMARY:Simulations of absorption in the brain of gamma quanta from posit ronium atoms DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-284@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Agata Jędruszczak ()\nAgata Jędruszczak for the J- PET collaboration\nagatajedruszczak1999@gmail.com\nJagiellonian University in Kraków\; Faculty of Physics\, Astronomy and Applied Computer Science\ , Profesora Stanisława Łojasiewicza 11\, 30-348 Kraków \n\nThe poster s hows the results of the research on the absorption in the brain of gamma q uanta from positronium atoms created during the PET imaging. \nPositronium imaging [1] is a new imaging method that allows to determine not only the location of the tumor\, but also the degree of its malignancy [2]. It is the multi-photon imaging\, which uses not only 2𝜸\, but also 3𝜸 anni hilations. Amount of detected photons from decay into 2𝜸 or 3𝜸 give information about tissue structure. Moreover\, the 3𝜸/2𝜸 ratio allow s the description of neoplastic changes [3]. The brain in these studies is approximated by a sphere with water. Monte Carlo simulations of positron decays and photon absorption in the brain and skull were performed. The si mulation results were compared with theoretical calculations. The results of the percent events for which none of photons scattered in the head are as follows: 26.10 ± 0.05 % for para-positronium and 8.40 ± 0.03 % for or tho-positronium (absorption in the brain)\, 20.84 ± 0.05 % for para-posit ronium\, 5.46 ± 0.02 % for ortho-positronium (absorption in the brain and in skull). The values of the ratio from the simulation are: 0.322 ± 0.00 2 for absorption in the brain and 0.262 ± 0.002 for absorption in the bra in and skull. The dependence of absorption probability of photons in the h ead on the location of positronium atom decay in the brain is determined. \nThe poster will present the above-mentioned results and plots obtained i n the simulations. The methods by which the simulation results were obtain ed will also be presented.\nReferences\n[1] P. Moskal et al.\, "Positroniu m imaging with the novel multi-photon PET scanner"\, Science Advances (in press) \n[2] P. Moskal\, D. Kisielewska\, C. Curceanu et al.\, "Feasibil ity study of the positronium imaging with the J-PET tomograph"\,Phys. Med. Biol. 64:055017 (2019)\n[3] B. Jasińska\, P. Moskal\, "A New PET Diagnos tic Indicator Based on the Ratio of 3𝜸/2𝜸 Positron Annihilation"\,Ac ta Phys. Pol. B\,48:1577-1582 (2017).\n\nhttps://indico.koza.if.uj.edu.pl/ event/4/contributions/284/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/284/ END:VEVENT BEGIN:VEVENT SUMMARY:Fast scanning of spent nuclear fuel dry storage casks using cosmic ray muons: Monte Carlo simulation study. DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-282@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Anzori Georgadze (Kiev Institute for Nuclear Researc h)\nStable development of fission-based nuclear energy facilities requires the safe management of spent nuclear fuel. The growing number spent nucle ar fuel in dry storage casks in intermediate storage sites around the worl d require efficient tools for non-destructive routine verifications of saf e storage of spent fuel assemblies. Using a cosmic ray muon for screening of spent nuclear fuel in dry storage casks appears to be the most suitable solution for non-destructive verifications of fuel casks for safeguard pu rposes. Fast scanning of a fully or partially loaded dry storage casks is evaluated using Monte - Carlo simulation with Geant4 package and muons pro duced using CRY event generator. The point of closest approach (POCA) algo rithms is used for reconstruction of muon interactions with dry storage ca sks. A Kolmogorov–Smirnov test was used to classify generated data sampl es for fully loaded casks and samples with one fuel assemble missing in dr y storage casks. We use the Receiver Operating Characteristic (ROC) techni que to characterize tradeoff between detection and false alarm rates. For one-hour measurement time detection rate can be achieved ~96%. The develop ed method of statistical analysis of reconstructed POCA points allows dete cting dry storage casks with one fuel assemblies missing in a relatively s hort time of ~1 hour without full image reconstruction. The results of mod elling demonstrate that the scattered muon tomography allows to perform ef ficient non-destructive scanning of dry storage casks for nuclear nonproli feration purposes.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributio ns/282/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/282/ END:VEVENT BEGIN:VEVENT SUMMARY:Physicochemical characteristic of poly(amidoamine) dendrimers are their application in controlled drug delivery systems DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-280@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Szota (Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences)\nPoly(amidoamine) (PAMAM ) dendrimers are monodisperse synthetic polymers with nanosize ranging fro m 1-14nm. Dendrimer synthesis can be precisely controlled in size\, shape\ , molecular mass\, composition\, and reactivity [1\,2]. The study's main a im was to investigate the correlation between the physicochemical properti es of the carrier and the active substance and the efficiency of the PAMAM -5FU complex formation. Experimental studies show that analysis of physico chemical properties of both PAMAM dendrimers and 5-fluorouracil play a sig nificant role in the formation of high-efficiency PAMAM-5FU complex. The l igand binding's effectiveness to the dendrimers’ structure is strictly d ependent on the complex formation conditions: molar ratio\, ionic strength \, pH\, and dendrimer generation. The fact that drug molecules bind most e ffectively under alkaline conditions when the dendrimer is close to the is oelectric point indicates the significant influence of the ligand charge\, which occurs in a deprotonated form. Studies have confirmed the system's ability to attach approximately 20 5FU molecules per dendrimer molecule fo r the fourth generation dendrimer and about 25 molecules for the sixth gen eration dendrimer. Comparing these values with the nominal number of amine groups present in the dendrimer structure\, a system efficiency of 16% fo r G4PAMAM and 5% for G6PAMAM dendrimers was obtained.\nThe decrease in the zeta potential of the PAMAM-5FU systems compared to the dendrimer itself indicates a change in the carrier's surface charge by drug immobilization. In addition\, it may reveal the presence of ligand molecules on the PAMAM surface. H1 NMR spectra indicate the presence of drug molecules both insi de the structure and on its surface. The research confirms the possibility of immobilizing the active agent in two ways and thus indicates the uniqu e properties of the structure of dendrimers.\nWe demonstrated that both G4 PAMAM and G6PAMAM present no toxicity towards normal cells. Furthermore\, the observed activity of 5-FU/PAMAM complexes in four cancer cell lines\, resulting in decreasing of a fluorouracil IC50 dose by up to 30%. Consider ing that most of the traditionally administered 5-FU is decomposed to inac tive metabolites before reaching its target\, drug conjugation with dendri mers seems to be a promising approach that increases drug toxicity and sta bility\, ultimately leading to overcoming of transportation-related drug r esistance.\n\nAcknowledgments: This work was partially supported by projec t NCN OPUS 2016/23/B/02788\,\nReferences\n1. Jachimska B. Physicochemical characterization of PAMAM dendrimer as a multifunctional nanocarriers\, Na noparticles in Pharmacotherapy. \; 2019. doi:10.1016/b978-0-12-816504-1.00 003-\n2. Rae JM\, Jachimska B. Analysis of dendrimer-protein interactions and their implications on potential applications of dendrimers in nanomedi cine. Nanoscale. 2021\;13(4):2703-2713. doi:10.1039/d0nr07607d\n\nhttps:// indico.koza.if.uj.edu.pl/event/4/contributions/280/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/280/ END:VEVENT BEGIN:VEVENT SUMMARY:The use of x-ray volume imaging system for verification of the pos itioning accuracy during stereotactic radiotherapy of the head and lungs DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-277@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Katarzyna Matusiak (AGH University of Science and Te chnology\, Al. Mickiewicza 30\, 30-059 Krakow)\nThe aim of this study was to analyzed the differences between the reconstruction of the patient's an atomy in the therapeutic area\, performed with the use of the X-ray Volume Imaging system (XVI) of the Elekta system\, and the actual patient positi oning. The results obtained from the cone beam tomography (CBCT) performed twice (before and after irradiation) were compared with the reference ima ges from computed tomography (CT) obtained during the treatment planning. \n\nThe comparison was made for two groups: 20 patients irradiated in the head area and 45 patients irradiated in the lung area. The results were an alyzed in three steps. The first was the analysis of data using the Studen t's t-test for one sample. It consisted in assessing whether the mean valu es of the isocenter shift implemented in relation to the isocentre planned in the direction of X\, Y\, Z for the studied patients are statistically significantly different from 0. Then the data were analyzed using the Stud ent's t-test for paired samples. It was done to check whether the mean val ues of the isocenter shift realized in relation to the isocentre planned i n the X\, Y\, Z directions for the studied patients before irradiation are statistically significantly different from the mean values of the shift f or the studied patients after irradiation. The third stage of the analysis of the results originating from the XVI system was the calculation of pop ulation systematic and random errors in order to calculate the CTV-PTV mar gins according to the van Herk method. The practical part of this work was carried out in cooperation with the Department of Radiotherapy for Childr en and Adults of the University Children's Hospital in Krakow.\n\nAnalysis of obtained data confirmed the high precision of radiotherapeutics proced ures performed at the Department of Radiotherapy for Children and Adults\, University Children's Hospital in Krakow. Moreover\, the effectiveness of XVI system in set-up margins reduction was confirmed.\n\nhttps://indico.k oza.if.uj.edu.pl/event/4/contributions/277/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/277/ END:VEVENT BEGIN:VEVENT SUMMARY:Free radicals influence on the positronium lifetime in melanocytes and melanomas cell cultures DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-269@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Ewelina Kubicz (Jagiellonian University)\nPositroniu m\, a bound state of positron and electron has been proposed as a novel bi omarker for examining cancer cells [1]. This atom is copiously created in cells during Positron Emission Tomography (PET) imaging [2-3].Our pre-clin ical studies have shown significant differences in the lifetime of positro nium between normal and neoplastic cells and tissues [4-5]. Due to the con version process concentrations of free radicals\, especially reactive oxyg en species (ROS) have a significant influence on the properties of positro nium\, such as its lifetime and production intensity in the tissue [6-7]. We investigated the role of antioxidants\, such as vitamin C and epigalloc atechin gallate (EGCG)\, on the values of the newly proposed biomarker. \n Studies were conducted on in vitro cell culture of normal human cell: mel anocyte HEMa-LP cell line and two cell lines of melanoma: WM115 (primary m elanoma) and WM266-4 (metastatic melanoma) as an example of cancer cells w ith different degree of malignancy. Cells were exposed to vitamin C in var ious concentrations (100\, 1000 µM) and EGCG (10\, 100 µM). Positronium lifetime was determined by means of Positron Annihilation Lifetime Spectro scopy and Na-22 isotope was used as a source of positrons. \n Obtained res ults showed differences in positronium lifetime\, between normal and cance r cell in relation to their malignancy. Resulting o-Ps lifetime in HEMa-LP \, WM115\, and WM266-4 cells was equal to 1.91(02)ns\, 1.95(03)ns\, 1.99(0 1)ns\, respectively in control\; 1.93(02)ns\, 1.96(01)ns\, 1.98(01)ns in 1 000 µM concentration of vitamin C and 1.91(02)ns\, 1.93(01)ns\, 1.89(02)n s in 100µM concentration of EGCG. No significant differences were observe d in measured solutions without the cells\, resulting in o-Ps lifetime of 1.91(02)ns\, 1.88(01)ns in vit. C and EGCG solution\, respectively.\n Outc ome of our experiment confirmed the validity of employing positronium as a n indicator\, which may have a direct impact on better and more accurate d iagnostics. The Jagiellonian Positron Emission Tomography scanner can be a pplied for simultaneous PET and positronium imaging [8-12].\n\nReferences: \n[1] Moskal\, P. et al.\, Patent No: US 9851456\; PL 227658\; PCT/EP2014 /068374.\n[2] Moskal\, P.et al.\, Nat. Rev. Phys. 1\, 527–529 (2019).\n [3] Moskal\, P. 2019 IEEE Nucl. Scien. Sympo. and Medical Imaging Conferen ce Proceedings\, NSS/MIC 2019\, doi:10.1109/NSS/MIC42101.2019.9059856 (20 20).\n[4] Moskal\, P. et al.\, Developing a Novel Positronium Biomarker fo r Cardiac Myxoma Imaging\, bioRxiv\n[5] Kubicz\, E. AIP Conf. Proc. 2182\, 050004 (2019).\n[6] Stepanov\, P. S. et al.\, Phys. Chem. Chem. Phys. 22 \, 5123–5131 (2020).\n[7] Shibuya\, K. et al. Commun. Phys. 3\, 173 (20 20).\n[8] Moskal\, P. et al. Phys. Med. Biol. 61\, 2025–2047 (2016).\n[9 ] Gajos\, A. et al. Adv. High Energy Phys. ID 8271280 (2018).\n[10] Moskal \, P. et al. Phys. Med. Biol. 64\, 055017 (2019).\n[11] Moskal\, P. et al. EJNMMI Phys. 7\, 44 (2020).\n[12] Moskal\, P. & Stępień\, E. PET Clin. 15\, 439–452 (2020).\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contrib utions/269/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/269/ END:VEVENT BEGIN:VEVENT SUMMARY:Impact of PRRT with the use of 90Y/177LuDOTA-TATE to change of SUV s obtained in 68Ga-DOTA-TATE PET/CT in patients with neuroendocrine tumors – does the use of a theroanostic pair of radiopharmaceuticals may affec t the estimation of survival after PRRT? DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-271@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Marta Opalińska (University Hospital in Krakow)\nIm pact of PRRT with the use of 90Y/177LuDOTA-TATE to change of SUVs obtained in 68Ga-DOTA-TATE PET/CT in patients with neuroendocrine tumors – does the use of a theroanostic pair of radiopharmaceuticals may affect the esti mation of survival after PRRT? \n\nIntroduction\nPeptide receptor radionuc lide therapy (PRRT) is an effective therapeutic option for metastatic neur oendocrine tumor (NET) therapy in case of good somatostatin receptor expre ssion in tumors tissue. Despite significant progress in management of NETs \, searching for novel predictive and prognostic factors is crucial. The h igh heterogeneity of the somatostatin receptors density in different NET m etastatic lesions and inside single tumours probably influence an clinical outcome. Some up-to-date studies indicate that the response to PRRT asses sed on the basis of imaging of somatostatin receptors may be a potentially useful tool for prediction of overall PRRT effect. \n\nAim\nAssessment of corrected SUV max change in metastatic NET lesions associated with PRRT c ounted in [68Ga]Ga-DOTA-TATE PET/CT and its potential impact on long-term treatment outcomes. \n\nMaterials and Methods\nAmong all patients treated with PRRT using 177Lu or 177Lu/90YDOTA-TATE in 2017-2019 due to disseminat ion of G1 and G2 classifications neuroendocrine neoplasm\, 13 patients who had 68Ga-DOTATATE PET/CT performed no longer than 6 months before and 6 m onths after PRRT. For all measurable metastatic lesions corrected SUVmax ( taking into account individual for each patients SUV max of reference orga ns normal liver or spleen)\, mean value of SUV max in both PET/CTs (before and after PRRT) was calculated. Those results were correlated with clinic al outcome of the disease assessed during follow-up one on the basis of ot her imaging studies as positive (stabilization (SD) or regression (PR)) or negative (progression (PD).\n \nResults\nThe mean follow-up was 8.9months . PD was found in patients\, PR or SD in 10 patients. Among patients with regression\, a decrease in the mean value of corrected SUVmax in comparis on to the baseline study of 277.12% was observed. Among patients with SD\, a mean of corrected SUVmax in comparison to the baseline study decreased by 180.80%. Decrease in the mean value of corrected SUVmax in comparison t o the baseline study in patients with regression and stabilization taken t ogether was in average 209.85% Increased values were observed among progre ssive patients\, where change of corrected SUVmax was in average 6.11%. \n \nConclusion\nA decrease in the value of corrected SUVmax in metastatic le sions obtained from routine PET/CT tests with 68Ga-DOTA-TATE may indicate a lower risk of neuroendocrine tumor progression within a 9 months from th e end of PRRT and may constitute an additional independent parameter helpi ng to estimate the risk of progression in this group of patients.\n\nhttps ://indico.koza.if.uj.edu.pl/event/4/contributions/271/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/271/ END:VEVENT BEGIN:VEVENT SUMMARY:Cyclotron produced gallium-68 chloride [68Ga]GaCl3 as an alternati ve to 68Ge/68Ga generators DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-265@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Jakub Boratyński (Univeristy of Warsaw)\, Michał J agodziński (University of Warsaw)\n**Jakub Boratyński 1\,2\, Michał Jag odziński 1\,2 and Zbigniew Rogulski 2**\n\n*1 Centrum Produkcji Radiofarm aceutyków VOXEL S.A\, ul. Wrocławska 1-3\, 30-006 Kraków\,\n2 Wydział Chemii\, Centrum Nauk Biologiczno-Chemicznych\, Uniwersytet Warszawski\, u l. Żwirki i Wigury 101\, 02-089 Warszawa*\n\n**E-mail: j.boratynski@voxel .pl**\n \nThe 68Ga isotope is usually eluted from the 68Ge/68Ga generator and is therefore readily available in PET (Nuclear Medicine) laboratories which do not have a cyclotron in place. The characteristics of the 68Ga is otope that this possesses make it a desirable radionuclide for PET diagnos tics and the first widely available PET radioactive metal ion for routine use worldwide. With the help of a chelator\, it can be easily attached to a biologically active molecules\, which makes it suitable for conjugation with various biomolecules using bifunctional chelators and various macromo lecules. Additionally\, the selection of the chelator enables one compound to be radiolabelled with different radiometals. Thanks to this\, it is po ssible to widely use (PET\, SPECT\, MRI\, multimodal PET/SPECT/CT and ther apy) of the compound only through the exchange of the radiometal with mini mal changes in biological behavior. This facilitates patient-centered care \, from diagnosis to molecular imaging to treatment\, e.g. possible combin ation with 177Lu or 90Y isotopes as a theranostic pair.\nDue to serious di sadvantages of 68Ge/68Ga generators\, such as a very high purchase cost\, short expiry date of the generator\, low activity of the obtained isotope\ , low availability on the market and the need to keep a break between succ essive elutions\, the number of PET studies with the use of 68Ga based rad iopharmaceuticals do not meet the market demand [1]\, hence recently attem pts have been made to obtain this valuable isotope using medical cyclotron s by irradiating of liquid (solution of 68Zn salts) [2] or solid target ma de of metallic 68Zn [3][4].\nThe paper presents the method of obtaining [6 8Ga]GaCl3 via a solid target technology in a medical cyclotron at the VOXE L Radiopharmaceuticals Production Center in Kraków\, in quality compliant with the requirements of the European Pharmacopoeia and Good Manufacturin g Practice (GMP). This method leads to obtain much greater activities\, al lowing for the subsequent distribution of the 68Ga isotope. The above meth od may be an attractive alternative to 68Ge/68Ga generators and in the fut ure\, by increasing the availability of the 68Ga isotope\, may contribute to changing the cancer diagnosis strategy.\n\n\n[1] K. Kumar\, Cancer Biot herapy And Radiopharmaceuticals\, 35\, 3 (2020).\n[2] F. Alves\, V.H.P. Al ves\, S.J. Do Carmo\, A.C. Neves\,M. Silva\, A.J. Abrunhosa\, Modern Physi cs Letters A\, 32\, 17\, (2017).\n[3] M. Lin\, G.J. Waligorski and C.G. Le pera\, Applied Radiation and Isotopes\, 133\, 1–3 (2018).\n[4] A.H. Alna hwi\, S. Tremblay\, S. Ait-Mohand\, J.F. Beaudoin\, B. Guéri\, Applied Ra diation and Isotopes\, 156\, 109014\, (2020).\n\nhttps://indico.koza.if.uj .edu.pl/event/4/contributions/265/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/265/ END:VEVENT BEGIN:VEVENT SUMMARY:Spectrometric study of biomolecular differences of β-cell EVs sub populations from hyperglycemic conditions DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-261@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Marzec (Jagiellonian University)\nExtracel lular vesicles (EVs) are a spherical shape structures surrounded by a prot ein-lipid membrane. EVs are important in the detection of various diseases \, such as cardiovascular disease or cancer\, and may have a high therapeu tic potential [1]. The molecular composition and size of EVs membranes var y depending on the source cells\, the current stage of development and env ironmental conditions. Their basic classification distinguishes three subp opulations: exosomes derived from endosomes (50–150 nm)\, microbubbles b udding in the cell membrane (100–1000 nm) and apoptotic bodies (100–50 00 nm). Specific features of these EVs subgroups have been proposed\, but there are still no standardized markers to distinguish these populations [ 2]. \nIn the work\, we propose the use of secondary ion mass spectrometry with a time-of-flight analyzer (ToF-SIMS) to assess the differences in the molecular composition of EV subpopulations: exosomes\, ectosomes and a mi xture of both populations. EVs\, derived from pancreatic β-cells grown un der hyperglycemia conditions (HC)\, were purified by Low-Vacuum Filtration and concentrated by ultracentrifugation. ToF-SIMS\, as a highly sensitive qualitative technique\, made it possible to perform a comparative analysi s of the tested samples. During the analysis\, significant differences in the intensities of the characteristic peaks of amino acids and individual lipid groups were revealed. The demonstrated changes concern EV subpopulat ions and their mixture obtained from glycerin conditions. It can be assume d that various EV subpopulations derived from pancreatic β-cell cultures are characterized by a changed molecular composition related to biogenesis of the discussed structures. The external environment has a significant i mpact on the protein-lipid EV membrane composition.\nKeywords: extracellul ar vesicles\; β-cell\; ToF-SIMS\; hyperglycemia\; lipidomic\;\n[1] E. St ępień\, E. Stankiewicz\, J. Zalewski\, J. Godlewski\, K. Zmudka\, and I. Wybrańska\, “Number of microparticles generated during acute myocardia l infarction and stable angina correlates with platelet activation.\,” Arch. Med. Res.\, vol. 43\, no. 1\, pp. 31–35\, Jan. 2012\, doi: 10.1016 /j.arcmed.2012.01.006.\n[2] E. Ł. Stępień\, A. Kamińska\, M. Surman\, D. Karbowska\, A. Wróbel\, and M. Przybyło\, “Fourier-Transform InfraR ed (FT-IR) spectroscopy to show alterations in molecular composition of EV subpopulations from melanoma cell lines in different malignancy\,” Bioc hemistry and biophysics reports\, vol. 25. Department of Medical Physics\, Marian Smoluchowski Institute of Physics\, Faculty of Physics\, Astronomy and Applied Computer Science\, Jagiellonian University\, 30-348\, Kraków \, Poland.\, p. 100888\, 2021\, doi: 10.1016/j.bbrep.2020.100888.\nThis wo rk was supported by the National Science Center (NCN)\, grant OPUS 17 to p rof. E. Stępień (No. 2019/33/B/NZ3/01004) and the SciMat Priority Resear ch Area budget under the Strategic Program Excellence Initiative at the Ja giellonian University.\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contrib utions/261/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/261/ END:VEVENT BEGIN:VEVENT SUMMARY:Comparison of SP3 and S-Trap LC-MS/MS approaches in proteomic anal ysis of ectosomes derived from thyroid cancer and normal thyroid follicula r cells DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-257@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Magdalena Surman (Jagiellonian University in Krakow\ , Faculty of Biology\, Institute of Zoology and Biomedical Research)\nThe small size of ectosomes makes their isolation and obtaining the appropriat e protein yield for liquid chromatography-tandem mass spectrometry (LC-MS/ MS) proteomic analyzes a considerable methodological challenge. Especially isolation of ectosomes from limited amount of body fluids of cancer patie nts means that a much smaller amount of protein is available for LC-MS/MS. The SP3 (solid-phase-enhanced sample preparation) method used by us so fa r [1] works when it is possible to obtain the appropriate amount of ectoso mal protein as a result of scaling cell cultures. The aim of this research was to develop a method of sample preparation for LC-MS/MS based of S-Tra p microcolumn technique that would give the same quality results despite u sing less protein.\n\nTwo cell lines were used in these research: anaplast ic thyroid carcinoma (8305C) and normal thyroid follicular (Nthy-ori 3-1) cells. Ectosomes were isolated from conditioned media concentrated by low- vacuum filtration by differential centrifugation\, and prepared for LC-MS/ MS using SP3 or S-Trap techniques. Then LC-MS/MS was used to analyze the p rotein content of the ectosome proteome. Next\, Gene Ontology (GO) analysi s was performed using UniProt Database to classify identified proteins acc ording to the biological processes\, molecular functions and their cellula r origin.\n\nUsing the SP3 method we identified 410 proteins in 8305C ecto somes and 558 proteins in Nthy-ori 3-1 ectosomes. S-Trap technique increas ed the numbers of identified proteins to 915 in 8305C ectosomes and to 804 proteins in Nthy-ori 3-1 ectosomes. For 8305C and Nthy-ori 3-1 ectosomes \, 304 and 357 proteins were identified by both protocols \, respectively. Alongside the proteins identified regardless of chosen sample preparation method (SP3 or S-Trap) provided significant number of protein that were n ot identified by the other one. According to GO the most abundant groups o f proteins for both types of ectosomes were those connected with cytosolic or membrane origin. In 8305C ectosomes\, several cancer-associated protei ns were found\, which suggests their possible role in cancer promotion.\n\ nAcknowledgements: The research was funded by the BioS Priority Research A rea under the program “Excellence Initiative –Research University” a t the Jagiellonian University in Krakow (U1U/P03/DO/13.17).\n\n[1] Surman et al.\, \, Int J Mol Sci. 2020\, 21(8): 2934.\n\nhttps://indico.koza.if.u j.edu.pl/event/4/contributions/257/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/257/ END:VEVENT BEGIN:VEVENT SUMMARY:Positronium biomarker in 3D melanoma spheroid model\, a novel prob e for cancer diagnosis DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-254@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Hanieh Karimi (Department of Medical Physics\, M. Sm oluchowski Institute of Physics\, Faculty of Physics\, Astronomy and Appli ed Computer Science\, Jagiellonian University\, Kraków\, Poland\, Total-B ody Jagiellonian-PET Laboratory\, Jagiellonian University\, Poland)\nSpher oids are three-dimensional cancer cell models able to mimic important prop erties of real tumors such as physical structure\, physiological character istics\, and gene expression patterns. In this research\, the lifetime of Positronium has been evaluated in spheroids formed from human melanoma cel l lines\, WM266-4 and WM115. In the first step\, spheroids were formed fro m WM266-4 and WM115 melanoma cell lines\, using the hanging drop method an d the size\, rate of proliferation and viability of spheroids were evaluat ed precisely by optical\, fluorescent Microscopy and micro-CT [1]. \nAfter precise determination of spheroid characteristics\, we created spheroids in 5D microplates for measuring positronium lifetime by PALS spectroscopy. The lifetime of positronium is environmentally dependent and it provides information about the size of intra-molecular spaces in cells\, thus it is related to the tissue morpholo-gy. To determine the positronium lifetime\ , the spheroids were inserted into an Aluminium chamber and irradi-ated wi th positrons emitted from 22Na radionuclide. The photons resulting from th e annihilation of positrons inside the spheroids were measured by the dedi cated detector build from BaF2 scintillators and digitizing acqui-sition s ystem. We observe differences in the lifetime of positronium depending on the degree of malignancy of the melanoma cells. WM266-4 showed a higher ve locity in division than WM115 which got 1.5 and 2.74-fold more cells after the 4th and 8th day while WM115 demonstrated 1.4 and 1.7-fold more cells after 4th and 8th days\, respectively. The Lifetime of o-Ps in WM266-4 sph eroids was 1.87 ns and 1.86 ns in 4th and 8th day after cul-turing while i n WM115 spheroids\, the o-Ps lifetime was 1.90 ns and 1.87 ns in 4th and 8 th day\, respectively. In conclusion\, both cell lines showed a reduction in an o-Ps lifetime during the time. This decrease in lifetime indicates t he reduction in molecular mobility because of the high concentration of ce lls in spheroids which are growing during the time. We can also consider t his difference in an o-Ps lifetime for malignancy diversity. The results w ill be reported in the context of its application of positronium as a biom arker for the in-vivo assess-ment of the degree of cancer malignancy with the total-body PET scanners [2].\nKeywords: Spheroids\, melanoma\, Hypoxia \, Positron imaging\nAcknowledgments\nThis work was supported by the Found ation for Polish Science (FNP) through grant TEAM/2017-4/39 program\, and DSC grant\, nom. N17/MNS/000023.\nReferences\n1. Karimi\, Hanieh\, et al. "X-ray microtomography as a new approach for imaging and analysis of tumor spheroids." Micron 137 (2020): 102917.\n2. Moskal\, Paweł\, and Ł. Stę pień\, Ewa. "Prospects and clinical perspectives of total-body PET imagin g using plastic scintillators." PET clinics 15.4 (2020): 439-452.\n\nhttps ://indico.koza.if.uj.edu.pl/event/4/contributions/254/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/254/ END:VEVENT BEGIN:VEVENT SUMMARY:Event Identification in Compton Camera Imaging via Machine Learnin g for Proton Therapy Monitoring DTSTART;VALUE=DATE-TIME:20211010T141000Z DTEND;VALUE=DATE-TIME:20211010T143000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-253@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Majid Kazemi Kozani (Marian Smoluchowski Institute o f Physics\, Jagiellonian University\, Kraków\, Poland)\nOne of the most i mportant challenges in hadron therapy is the development of online monitor ing techniques. Monitoring systems based on the detection of secondary rad iation such as prompt gamma (PG) emission produced during treatment are pr omising approaches for this purpose [1\,2]. The SiFi-CC\, a Compton camera based on stacks of heavy scintillating fibers and SiPMs\, is being develo ped for PG imaging [3\,4]. A machine learning approach based on TMVA [5] t o recognize Compton events is proposed for the classification of pseudo-da ta generated by the Geant4 simulation for a 180 MeV spot-scanning proton b eam impinging on a PMMA phantom. To reconstruct a Compton event\, a minimu m of two interactions is required. Therefore\, the proposed method first b uilds a learning set of the events filtered with interactions that yielded at least one interaction in each of two modules of the SiFi-CC. The data set is used to train the boosted decision tree (BDT) model using nine feat ures including the position and deposited energy of interactions in the sc atterer and the absorber\, and the cosine of internal scattering angles te rm. A 10-fold cross-validation of the BDT model shows a great increase in the signal to background ratio. A software based on the LM-MLEM algorithm [6\,7] was applied for the reconstruction of the PG distribution. Very goo d agreement between the reconstructed distal edge position and that of sim ulated Compton events was obtained. Moreover\, it was shown that the preci sion of a few millimeters in distal edge position determination is feasibl e.\nReferences:\n[1] C.H. Min\, et al.\, Nucl. Instrum. Methods Phys. Res. A. vol. 580\, 2007\, pp. 562–565.\n[2] L. Kelleter\, et al.\, Phys. Med .\, vol. 34\, 2017\, pp. 7-17.\n[3] J. Kasper\, et al.\, Phys. Med.\, vol. 76\, Jul. 2020\, pp. 317-325.\n[4] A. Wrońska\, et al.\, Acta. Phys. Pol . B\, vol. 51\, 2020\, pp. 17-25.\n[5] A. Hoecker\, et al.\, Rep. no. CERN -OPEN-2007-007.\n[6] S. J. Wilderman\, et al.\, IEEE Nucl. Sci. Symp. Med. Imaging Conf. Rec.\, vol. 3\, 1998\, pp. 1716-1720.\n[7] S. J. Wilderman\ , et al.\, IEEE Trans. Nucl. Sci.\, vol. 48\, 2016\, pp. 111-116.\n\nhttps ://indico.koza.if.uj.edu.pl/event/4/contributions/253/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/253/ END:VEVENT BEGIN:VEVENT SUMMARY:The in vitro study of the toxicity and therapeutic effects of iron oxide nanoparticles with different core size DTSTART;VALUE=DATE-TIME:20211010T140000Z DTEND;VALUE=DATE-TIME:20211010T142000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-248@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Natalia Janik-Olchawa (Faculty of Physics and Applie d Computer Science\, AGH University of Science and Technology)\nNanotechno logy is a combination of science\, engineering and technology in a nanosca le. This revolutionary technics is used in many fields of science and life [1\,2]. Advances in nanotechnology resulted in the development of therano stic nanoparticles constituting the diagnostic and therapeutic agent in a single particles [3]. Such features are possessed\, among others\, by magn etite nanoparticles which serving as the contrast agents for MRI can be us ed in medical diagnostics. In turn\, their interaction with the external m agnetic field cause that they can be used as drug carries or agents for th e local hyperthermia.\n To introduce iron oxide nanoparticles (IONPs) in to clinical practice their biocompatibility and potential mechanisms of to xicity are to be determined. The main objective of our study was the analy sis of therapeutic potential and potential toxicities of IONPs based on di fferent cellular models. The impact of PEG-coated magnetite NPs with three different core diameters (5\, 10 and 30 nm)\, determined by TEM\, on the normal and cancer cell lines was examined. The cytotoxicity of nanomateria ls was assessed by MTT assay and trypan blue staining. Cell motility\, int racellular ROS production and actin cytoskeleton rearrangements were also studied. For this purpose\, fluorescence microscopy and time-lapse videomi croscopy were used. Moreover\, the anomalies in the distribution and struc ture of biomolecules induced in cells by IONPs were examined and for this purpose Raman microspectroscopy was applied.\n The obtained results show ed changes in cell life parameters which depended on the IONPs core diamet er\, cell line\, exposure time and dose. What is more\, the fluorescence m icroscopy with TIRF module showed changes in cytoskeleton organization and cell morphology for some cell lines after the treatment with IONPs.\n\nRe ferences:\n[1] Dadfar SM\, Roemhild K\, Drude NI\, von Stillfried S\, Knü chel R\, Kiessling F\, Lammers T. Iron oxide nanoparticles: Diagnostic\, t herapeutic and theranostic applications. Adv Drug Deliv Rev. 2019. 138:302 -325.\n[2] Suciu M\, Ionescu CM\, Ciorita A\, Tripon SC\, Nica D\, Al-Sala mi H\, Barbu-Tudoran L. Applications of superparamagnetic iron oxide nanop articles in drug and therapeutic delivery\, and biotechnological advanceme nts. Beilstein J Nanotechnol. 2020. 11:1092-1109.\n[3] Revia RA\, Zhang M. Magnetite nanoparticles for cancer diagnosis\, treatment\, and treatment monitoring: recent advances. Mater Today (Kidlington). 2016. 19(3):157-168 .\n\nhttps://indico.koza.if.uj.edu.pl/event/4/contributions/248/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/248/ END:VEVENT BEGIN:VEVENT SUMMARY:Convolutional neural networks in classification of multi-photon co incidences in J-PET scanner DTSTART;VALUE=DATE-TIME:20211010T134000Z DTEND;VALUE=DATE-TIME:20211010T140000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-283@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Paweł Konieczka (NCBJ)\nPaweł Konieczka\, on behal f of the J-PET collaboration\n\nConvolutional Neural Networks are excellen t at analyzing images by\nlearning abstract representations. CNN has been an overwhelming strategy\nin computer vision tasks and has achieved expert -level performances in\nvarious fields. There has been a surge of interest in the potential of\nCNN among radiology researchers and several studies have already been\npublished in areas such as classification [1] and image reconstruction\n[2].\n\nFirst general methodology to transform a non-imag e data into an image\nfor CNN architectures has been presented in [3]. Nev ertheless\, this\nmethod cannot be applied to large data sets\, where numb er of features is\nvery small\, because of computational complexity of PCA . The introduction\nof scheme of non-image data transformation into 2-dime nsional matrices\nwill be proposed [4].\n\nThe goal of this poster is to p resent results of multi-photon\ncoincidences classification in J-PET scann er using CNNs. Bayesian\noptimization of two convolutional network archite ctures (DeepInsight\n[3]\, YOLOv1 [5]) will be presented.\n\nReferences:\n \n[1] Yasaka\, Koichiro\, et al. Deep learning with convolutional neural n etwork\nfor differentiation of liver masses at dynamic contrast-enhanced C T: a\npreliminary study. Radiology\, 2018\, 286.3: 887-896.\n\n[2] Liu\, F ang\, et al. Deep learning MR imaging--based attenuation correction\nfor P ET/MR imaging. Radiology\, 2018\, 286.2: 676-684.\n\n[3] Sharma\, Alok\, e t al. DeepInsight: A methodology to transform a non-image\ndata to an imag e for convolution neural network architecture. Scientific\nreports\, 2019\ , 9.1: 1-7.\n\n[4] Raczyński\, Lech\, Introduction of non-image PET data transformation to\nimage-form approach for classification using Convolutio nal Neural\nNetworks. 1st Symposium on Theranostics\, 2021.\n\n[5] Redmon\ , Joseph\, et al. You only look once: Unified\, real-time object\ndetectio n. In: Proceedings of the IEEE conference on computer vision and\npattern recognition. 2016. p. 779-788.\n\nE-mail: pawel.konieczka@ncbj.gov.pl\n\nh ttps://indico.koza.if.uj.edu.pl/event/4/contributions/283/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/283/ END:VEVENT BEGIN:VEVENT SUMMARY:CPT symmetry test in positronium annihilations with the J-PET dete ctor DTSTART;VALUE=DATE-TIME:20211010T132000Z DTEND;VALUE=DATE-TIME:20211010T134000Z DTSTAMP;VALUE=DATE-TIME:20260815T031001Z UID:indico-contribution-18-250@indico.koza.if.uj.edu.pl DESCRIPTION:Speakers: Neha Chug (Jagiellonian University)\nDiscrete symmet ry under the combined transformation of charge\, parity\, and time reversa l (CPT) can be tested in the decays of positronium atom\, the lightest bou nd system built of charged leptons.\nJagiellonian Positron Emission Tomogr aph (J-PET) device constructed from plastic scintillators\, detects the ph otons originating from electron positron annihilation. This feature enable s J-PET to study CPT symmetry in the three photon annihilations of the tri plet state of positronium. Signs of violation of the CPT symmetry can be s ought as a non-vanishing expectation value of an angular correlation opera tor that is odd under CPT transformation. Technique to estimate the spin of ortho-positronium and momenta of annihilation photons for single record ed ortho-positronium event allows J-PET to measure the expectation value o f CPT symmetry odd angular correlation operator. J-PET measures a broad ra nge of kinematical configurations of ortho-positronium annihilation to thr ee photons and is the first experiment to determine the full range of the CPT-odd angular correlation. The presentation will include the methods of performing the CPT symmetry test using an angular correlation operator whi ch involves the spin and momenta of photons originating from o-Ps${\\to}3{ \\gamma}$ decay using extensive size positronium production and annihilati on chambers with the J-PET detector.\n\nhttps://indico.koza.if.uj.edu.pl/e vent/4/contributions/250/ LOCATION:Theranostics Center / on-line URL:https://indico.koza.if.uj.edu.pl/event/4/contributions/250/ END:VEVENT END:VCALENDAR